Molecular detection of apicomplexan protozoa in Hokkaido brown bears (Ursus arctos yesoensis) and Japanese black bears (Ursus thibetanus japonicus)
Molecular detection of apicomplexan protozoa in Hokkaido brown bears (Ursus arctos yesoensis) and Japanese black bears (Ursus thibetanus japonicus)
复制标题
北海道棕熊（Ursus arctos yesoensis）和日本黑熊（Ursus thibetanus japonicus）顶端复门原生动物的分子检测
DOI：
10.1007/s00436-020-06873-3
复制
发表时间：
2020
影响因子：
2
通讯作者：
Tsubota Toshio
中科院分区：
文献类型：
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作者：
Moustafa Mohamed Abdallah Mohamed;Sasaki Ayaka;Shimozuru Michito;Nakao Ryo;Sashika Mariko;Yamazaki Koji;Koike Shinsuke;Tanaka Junpei;Tamatani Hiroo;Yamanaka Masami;Ishinazaka Tsuyoshi;Tsubota Toshio
许多蜱传病原体（TBP）存在于野生动物中。本研究的目的是揭示野生熊在维持TBP中的作用。采用反向线性杂交（RLB）和巢式PCR两种分子生物学方法，对来自北海道的49只棕熊（Ursus arctos yesoensis）、18只来自栃木县的日本黑熊（Ursus thibetanus）和66只来自长野县的日本黑熊进行了检测。共分离到5个TBP（熊肝虫、巴贝斯虫）。UR 2样群、Cytauxzoonsp. UR 1，巴贝西阿斯属。从熊血DNA样品中检测到UR 1和田鼠巴贝耳球虫（Bababellacemicroti）。首次从日本黑熊的血液DNA中检测到microtis，检出率为6.0%（5/84）。在检测到的病原体中，H. ursi，Babesiasp. UR 2类病原体和Cytauxzoonsp. UR 1被认为是熊中最常见的三种TBP。H.日本黑熊和巴贝氏asp.北海道棕熊的UR 2样组较高（89.8%比40.5%）。巴贝虫的流行。与长野黑熊（18.2%）相比，日本东北黑熊的UR 1基因表达显著增高（44.4%）。成年熊TBP检出率显著高于幼熊。本研究表明，日本熊种有助于在日本的几个TBP的传输。熊类分布的扩大可能会导致TBPs意外传播给人类和家畜。
Many tick-borne pathogens (TBPs) are present in wildlife. The objective of this study is to reveal the role of wild bears in maintaining TBPs. A total of 49 brown bears (Ursus arctos yesoensis) from Hokkaido, and 18 Japanese black bears (Ursus thibetanus japonicus) from Tochigi, and 66 Japanese black bears from Nagano were examined by two molecular methods, reverse line blot (RLB) hybridization, and nested PCR. A total of 5 TBPs (Hepatozoon ursi,Babesiasp. UR2-like group,Cytauxzoonsp. UR1,Babesiasp. UR1, andBabesia microti) were detected from bear blood DNA samples.B. microtiwas detected from blood DNA samples of Japanese black bear for the first time, with the prevalence of 6.0% (5/84). Out of detected pathogens,H. ursi,Babesiasp. UR2-like pathogens, andCytauxzoonsp. UR1 were considered as three of the most prevalent TBPs in bears. The prevalence ofH. ursiwere significantly higher in Japanese black bear (0% vs 96.4%) while that ofBabesiasp. UR2-like group was higher in Hokkaido brown bears (89.8% vs 40.5%). The prevalence ofBabesiasp. UR1 were significantly higher in Japanese black bears from Tochigi (44.4%), comparing with those from Nagano (18.2%). The prevalence of the detected TBPs were significantly higher in adult bears, comparing with those in younger bears. The present study suggests that Japanese bear species contribute in the transmission of several TBPs in Japan. The expanding distribution of bears might cause the accidental transmission of TBPs to humans and domestic animals.