Occurrence of copper proteins through the three domains of life: A bioinformatic approach
Occurrence of copper proteins through the three domains of life: A bioinformatic approach
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DOI：
10.1021/pr070480u
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发表时间：
2008-01-01
影响因子：
4.4
通讯作者：
Rosato, Antonio
中科院分区：
文献类型：
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作者：
Andreini, Claudia;Banci, Lucia;Rosato, Antonio
在高通量基因组水平的蛋白质研究工作中，如结构基因组学，金属结合性质的系统实验表征（即，金属蛋白质组的研究）并不总是被追求，并且仍然远非微不足道。在目前的工作中，我们已经应用了生物信息学的方法来调查发生（假定）铜结合蛋白在57个不同的生物跨越整个生命树。我们发现，铜蛋白质组的大小一般小于1%的生物体的总蛋白质组，在真核生物和原核生物。与锌结合蛋白和非血红素铁蛋白相比，铜结合蛋白的出现相对较少。这可能是由于生物利用度较差（特别是在古代世界的铁方面）和铜化学的复杂性及其相关风险，这可能对铜结合蛋白的自然选择产生不利影响。目前的分析表明，金属配位球和蛋白质功能之间有很强的关系。一个网络，涉及蛋白质的铜运输和呼吸的作用进行了鉴定，部分或全部的检测在大多数的生物体检查。
In high-throughput genome-level protein investigation efforts, such as Structural Genomics, the systematic experimental characterization of metal-binding properties (i.e., the investigation of the metal loproteome) is not always pursued and remains far from trivial. In the present work, we have applied a bioinformatic approach to investigate the occurrence of (putative) copper-binding proteins in 57 different organisms spanning the entire tree of life. We found that the size of the copper proteome is generally less than 1% of the total proteome of an organism, in both eukaryotes and prokaryotes. The occurrence of copper-binding proteins is relatively scarce when compared to that of zinc-binding proteins and of non-heme iron proteins. This may be due to both poorer bioavailability (in particular with respect to iron in the ancient world) and the complexity of copper chemistry and the risks associated with it, which may have adversely affected natural selection of copper-binding proteins. The present analysis shows that there is a strong relationship between the metal coordination sphere and protein function. A network involving proteins having roles in both copper transport and respiration was identified, parts or all of which are detected in the majority of the organisms examined.