GROWTH AND DIVISION OF SPIROPLASMAS - MORPHOLOGY OF SPIROPLASMA-CITRI DURING GROWTH IN LIQUID-MEDIUM
GROWTH AND DIVISION OF SPIROPLASMAS - MORPHOLOGY OF SPIROPLASMA-CITRI DURING GROWTH IN LIQUID-MEDIUM
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DOI：
10.1128/jb.147.2.642-652.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
BOVE, JM
中科院分区：
文献类型：
--
作者：
GARNIER, M;CLERC, M;BOVE, JM
螺旋支原体S.用一种新开发的转移技术，保留了有机体的螺旋形态，用EM检查了柑橘。最小的活细胞是2圈（基本）螺旋。在对数生长期，生物体的长度增加，并通过收缩分开，释放2圈基本螺旋。最常分裂的亲本螺旋是带有. apprx的螺旋。4圈，产生2个基本螺旋。pH值和温度对生物体形态的影响也进行了研究。在非缓冲培养基中，微生物的生长导致pH值显著降低，从而形成异常形态和细胞溶解。在37度。C，细胞分裂受到抑制，导致2-转角螺旋逐渐消失，其他螺旋的平均长度增加。最后，在生长周期的任何阶段，都从未见过螺旋从圆形物体中产生。
The helical mycoplasma S. citri was examined by EM with a newly developed transfer technique which preserves the helical morphology of the organism. The smallest viable cell was a 2-turn (elementary) helix. During the logarithmic phase of growth, organisms increased in length and divided by constriction, liberating 2-turn elementary helices. The most frequently dividing parental helix was one with .apprx. 4 turns, yielding 2 elementary helices. Influence of pH and temperature on the morphology of the organism was also investigated. In unbuffered medium, growth of the organism produced a significant decrease in pH and a consequent formation of abnormal morphological forms and cell lysis. At 37.degree. C, cell division was inhibited, leading to a progressive disappearance of 2-turn helices and an increase in the average length of other helices. Finally, helices were never seen to arise from round bodies at any stage of the growth cycle.