Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro
Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro
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DOI：
10.1016/j.bbrc.2006.05.014
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发表时间：
2006-07-07
影响因子：
3.1
通讯作者：
Yamamoto, Ken
中科院分区：
文献类型：
--
作者：
Furuno, Kenji;Masatsugu, Toshihiro;Yamamoto, Ken
SuZ12是一种多梳蛋白，与EED和组蛋白甲基转移酶EZH2形成多梳抑制复合体(PRC2/3)。虽然SUZ12在调节PrC2/3复合体活性中的重要作用已被证明，但该蛋白的额外功能仍被提出。在这里，我们证明了SUZ12在体外和体内都与WD-Repeat蛋白MEP50相互作用。我们发现MEP50选择性地与核心组蛋白中的组蛋白H2A结合，并介导蛋白质精氨酸甲基转移酶PRMT5的转录抑制，该酶已知甲基化H2A和H4。这些结果表明，SUZ12可能通过与MEP50的物理相互作用而在转录调控中发挥作用，MEP50可能是PRMT5与其底物H2A之间的适配器。(C)2006 Elsevier Inc.保留所有权利。
SUZ12 is a Polycomb group protein that forms Polycomb repressive complexes (PRC2/3) together with EED and histone methyltransferase EZH2. Although the essential role of SUZ12 in regulating the activity of the PRC2/3 complexes has been demonstrated, additional function of this protein was suggested. Here, we show that SUZ12 interacts with WD-repeat protein MEP50 in vitro and in vivo. We show that the MEP50 binds histone H2A selectively among core histones, and mediates transcriptional repression of protein arginine methyltransferase PRMT5, which is known to methylate H2A and H4. These results suggest that SUZ12 might have a role in transcriptional regulation through physical interaction with MEP50 that can be an adaptor between PRMT5 and its substrate H2A. (c) 2006 Elsevier Inc. All rights reserved.