Loss of Xist imprinting in diploid parthenogenetic preimplantation embryos
Loss of Xist imprinting in diploid parthenogenetic preimplantation embryos
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DOI：
10.1006/dbio.2001.0295
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发表时间：
2001-07-15
影响因子：
2.7
通讯作者：
Brockdorff, N
中科院分区：
文献类型：
--
作者：
Nesterova, TB;Barton, SC;Brockdorff, N
我们利用RNA FISH分析了Xist在孤雌生殖和控制受精前胚胎中的表达模式。在正常的XX胚胎中，母源性的Xist等位基因在着床前发育过程中受到抑制。父系等位基因早在2细胞阶段就表达了。在孤雌生殖胚胎中，我们观察到Xist RNA的表达和积累从桑葚胚期开始，表明母体印迹的丧失。在大多数细胞中，表达来自单个等位基因，表明X染色体计数发生以建立适当的单等位基因Xist表达。我们在印迹和随机X失活调节模型的背景下讨论这些数据。(C) 2001学术出版社。
We have analysed Xist expression patterns in parthenogenetic and control fertilised preimplantation embryos by using RNA FISH. In normal XX embryos, maternally derived Xist alleles are repressed throughout preimplantation development. Paternal alleles are expressed as early as the 2-cell stage. In parthenogenetic embryos, we observed Xist RNA expression and accumulation from the morula stage onwards, indicating loss of maternal imprinting. In the majority of cells, expression was from a single allele, indicating that X chromosome counting occurs to establish appropriate monoallelic Xist expression. We discuss these data in the context of models for regulation of imprinted and random X inactivation. (C) 2001 Academic Press.