Effect of N-(n-butyl) thiophosphoric triamide on urea metabolism and the assimilation of ammonium by Triticum aestivum L.
Effect of N-(n-butyl) thiophosphoric triamide on urea metabolism and the assimilation of ammonium by Triticum aestivum L.
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DOI：
10.1007/s10725-010-9513-6
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发表时间：
2011-01-01
影响因子：
4.2
通讯作者：
Maria Aparicio-Tejo, Pedro
中科院分区：
文献类型：
--
作者：
Artola, Ekhine;Cruchaga, Saioa;Maria Aparicio-Tejo, Pedro
尿素作为氮肥的使用量已经增加到如此程度，以至于它现在是世界上使用最广泛的肥料。然而，由于氨挥发导致的氮损失导致其效率降低，因此多年来已经开发了不同的方法来减少这些损失。最近的一种方法是将尿素与尿素酶抑制剂如N-（正丁基）硫代磷酰三胺（NBPT）结合使用，以试图延迟尿素在土壤中的水解。本研究旨在深入分析NBPT对植物生长和氮代谢的影响。在温室试验中培养小麦植株，持续4周，并施用不同浓度（0、0.012、0.062、0.125%）的尿素和NBPT。每个处理重复六次。还培养了未受精的对照。在生育末期分析了与氮代谢有关的几个参数。发现NBPT的使用具有可见的效果，例如在处理的第一周结束时叶尖的暂时变黄。在代谢水平上，发现用抑制剂处理的植物在其组织中具有更多的尿素，并且在研究期结束时具有较低的氨基酸含量、较低的谷氨酰胺合成酶活性以及较低的脲酶和谷氨酰胺合成酶含量，而其脲酶活性似乎在此阶段已经恢复。
The use of urea as an N fertilizer has increased to such an extent that it is now the most widely used fertilizer in the world. However, N losses as a result of ammonia volatilization lead to a decrease in its efficiency, therefore different methods have been developed over the years to reduce these losses. One of the most recent involves the use of urea combined with urease inhibitors, such as N-(n-butyl) thiophosphoric triamide (NBPT), in an attempt to delay the hydrolysis of urea in the soil. The aim of this study was to perform an in-depth analysis of the effect that NBPT use has on plant growth and N metabolism. Wheat plants were cultivated in a greenhouse experiment lasting 4 weeks and fertilized with urea and NBPT at different concentrations (0, 0.012, 0.062, 0.125%). Each treatment was replicated six times. A non-fertilized control was also cultivated. Several parameters related with N metabolism were analysed at the end of growth period. NBPT use was found to have visible effects, such as a transitory yellowing of the leaf tips, at the end of the first week of treatment. At a metabolic level, plants treated with the inhibitor were found to have more urea in their tissues and a lower amino acid content, lower glutamine synthetase activity, and lower urease and glutamine synthetase content at the end of the study period, whereas their urease activity seemed to have recovered by this stage.