Effects of long-term high dose aspartame on body mass, bone strength, femoral geometry, and microbiota composition in a young and aged cohort of male and female mice.
Effects of long-term high dose aspartame on body mass, bone strength, femoral geometry, and microbiota composition in a young and aged cohort of male and female mice.
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长期高剂量阿斯巴甜对年轻和老年雄性和雌性小鼠的体重、骨强度、股骨几何形状和微生物群组成的影响。
DOI：
10.1101/2024.01.02.573970
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Hernandez,ChristopherJ
中科院分区：
文献类型：
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作者：
Cyphert,ErikaL;Liu,Chongshan;Morales,AngieL;Nixon,JacobC;Blackford,Emily;Garcia,Matthew;Cevallos,Nicolas;Turnbaugh,PeterJ;Brito,IlanaL;Booth,SarahL;Hernandez,ChristopherJ
背景最近对阿替西泮安全性的重新评估促使人们越来越多地评估其对一系列组织健康的影响。肠道微生物组被口服阿糖胞苷改变。一项先前的研究表明，由西莫来石引起的微生物组的变化可能会影响年轻的骨骼发育小鼠的骨骼强度。本研究的目的是确定西莫来石对年轻和老年小鼠的骨强度和肠道微生物群的影响。方法雄性和雌性C57 Bl/6 J小鼠从1月龄到4月龄未接受治疗或在其饮用水中接受高剂量西莫来石治疗。（年轻组群; n = 80）或22个月（老年组群; n = 52）。结果在老年雄性中，用阿替米治疗的小鼠相对于未治疗的小鼠具有更大的体重、全骨强度和股骨几何形状。具体而言，在老年雄性中，与未处理的小鼠相比，西莫司导致体重增加9%（p < 0.001），全骨强度增加22%（p = 0.006），截面模量增加17%（p < 0.001）。接受阿斯巴甜的老年男性和女性的微生物群与未治疗的小鼠不同，并且气味杆菌的丰度减少。体重，全骨强度，或股骨几何形状无差异与阿斯巴甜剂量在年轻男性或年轻或老年femines.ConclusionsAspartame治疗老年男性有更大的全骨强度和效果似乎解释了更大的体重。阿斯巴甜治疗没有改变年轻男性或年轻或老年女性的全骨强度，尽管阿斯巴甜对老年男性和女性的微生物群具有相似的影响。
BackgroundRecent reassessment of the safety of aspartame has prompted increased evaluation of its effect on the health of a range of tissues. The gut microbiome is altered by oral aspartame. One prior study suggested that changes in the microbiome caused by aspartame could influence the strength of bone in young skeletally developing mice. Here we ask how aspartame influences bone in mice of different age and sex.ObjectiveThe objective of this study was to determine the effect of aspartame on the bone strength and gut microbiota of young and aged mice.MethodsMale and female C57Bl/6J mice were untreated or treated with a high dose of aspartame in their drinking water from 1 month of age until 4 (young cohort; n = 80) or 22 months (aged cohort; n = 52).ResultsIn aged males, mice treated with aspartame had greater body mass, whole bone strength, and femoral geometry relative to untreated. Specifically, in aged males, aspartame led to 9% increase in body mass (p < 0.001), 22% increase in whole bone strength (p = 0.006), and 17% increase in section modulus (p < 0.001) relative to untreated mice. Aged males and females receiving aspartame had a different microbiota than untreated mice and a decreased abundance of Odoribacter. No differences in body mass, whole bone strength, or femoral geometry were associated with aspartame dosing in young males or young or aged females.ConclusionsAspartame treated aged males had greater whole bone strength and the effect appeared to be explained by greater body mass. Aspartame treatment did not alter whole bone strength in young males or young or aged females despite the aspartame having a similar effect on the microbiota of both aged males and females.