REDUCTIVE METHYLATION - METHOD FOR PREPARING FUNCTIONALLY ACTIVE RADIOACTIVE RIBOSOMES
REDUCTIVE METHYLATION - METHOD FOR PREPARING FUNCTIONALLY ACTIVE RADIOACTIVE RIBOSOMES
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DOI：
10.1016/0014-5793(73)80429-6
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发表时间：
1973-01-01
期刊：
影响因子：
3.5
通讯作者：
CRICHTON, RR
中科院分区：
文献类型：
--
作者：
MOORE, G;CRICHTON, RR
放射性核糖体蛋白广泛应用于核糖体结构和功能的研究。标准的制备程序包括在放射性氨基酸源上培养细菌；一种昂贵且不易适用于高等生物的方法。本文描述了一种简单的标记30 S、50 S和70 S颗粒的所有核糖体蛋白而不影响其功能活性的方法。该方法涉及使用甲醛和硼氢化钠对赖氨酸残基进行还原甲基化[11]。用这种方法可以制备具有极高比放射性的核糖体。
Radioactive ribosomal proteins are widely used in many studies on ribosome topography and function. The standard preparative procedure involved growing bacteria on a radioactive amino acid source; a method which is expensive and not readily applicable to higher organisms.This paper describes a simple method for labelling all the ribosomal proteins of 30 S, 50 S and 70 S particles without affecting their functional activities. The method involves reductive methylation of lysine residues using formaldehyde and sodium borohydride [11. Ribosomes with extremely high specific radioactivity can be prepared in this manner.