Salvianolic acid B inhibits myofibroblast transdifferentiation in experimental pulmonary fibrosis via the up-regulation of Nrf2
Salvianolic acid B inhibits myofibroblast transdifferentiation in experimental pulmonary fibrosis via the up-regulation of Nrf2
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DOI：
10.1016/j.bbrc.2017.11.014
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Li, Yanchun
中科院分区：
文献类型：
--
作者：
Liu, Miao;Xu, Hanying;Li, Yanchun
丹参酚酸B（SalB）是从丹参中提取的最具生物活性的成分之一，其抗氧化能力与其对氧化应激引起的细胞损伤的保护作用相一致。本研究的目的是评估SalB对实验性肺纤维化的影响及其改善纤维化发病过程中氧化/抗氧化失衡的能力。SalB的抗纤维化活性首先在转化生长因子β 1（TGF-β 1）刺激的MRC-5细胞中得到证实。通过检测肝纤维化过程中ROS的产生、谷胱甘肽（GSH）和丙二醛（MDA）的水平，证实SalB对氧化应激的保护作用。Western blot和PCR检测结果表明，SalB在体外可上调核因子红细胞衍生2样蛋白2（Nrf 2）的表达，并诱导Nrf 2核转位，这可能是SalB抗肝纤维化的机制之一。此外，在BLM诱导的肺纤维化大鼠中证实了SalB的体内抗纤维化和抗氧化能力。免疫组化结果显示，Nrf 2在成纤维细胞灶（FF）区表达缺失，而SalB处理可增加Nrf 2在肺组织中的表达，尤其是在FF区。(C)2017爱思唯尔公司All rights reserved.
Salvianolic acid B (SalB) is one of the most bioactive components extracted from Salvia miltiorrhiza, and its antioxidant capacity corresponds with its protective effects against cell injury from oxidative stress. The aim of the present study was to evaluate the effect of SalB on experimental pulmonary fibrosis and its ability to ameliorate the oxidative/antioxidative imbalance during fibrosis pathogenesis. The anti fibrotic activity of SalB was first confirmed in Transforming growth factor beta 1(TGF-beta 1)-stimulated MRC-5 cells. The protection of SalB against oxidative stress during fibrogenesis in vitro was verified by detecting ROS production, the levels of glutathione (GSH) and malondialdehyde (MDA). The Western blot and PCR results indicated that SalB could up-regulate nuclear factor erythroid-derived 2-like 2 (Nrf2) at both the protein and mRNA levels and induce Nrf2 nuclear translocation in vitro, which may be the mechanism underlying the anti-fibrotic capacity of SalB. Furthermore, the anti-fibrotic and antioxidant capacities of SalB in vivo were confirmed in rats with BLM-induced pulmonary fibrosis. The immunohistochemistry results showed that Nrf2 was absent in fibroblastic foci (FF) areas, while the SalB treatment could increase the expression of Nrf2 in lung tissues, especially in FF areas. (C) 2017 Elsevier Inc. All rights reserved.