Basic fibroblast growth factor combined with biodegradable hydrogel promotes healing of facial nerve after compression injury: An experimental study
Basic fibroblast growth factor combined with biodegradable hydrogel promotes healing of facial nerve after compression injury: An experimental study
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DOI：
10.3109/00016480902896139
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发表时间：
2010-01-01
影响因子：
1.4
通讯作者：
Yamamoto, Masaya
中科院分区：
文献类型：
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作者：
Komobuchi, Hayato;Hato, Naohito;Yamamoto, Masaya
结论局部应用碱性成纤维细胞生长因子（bFGF）水凝胶促进创伤性面瘫更快愈合，这是由于bFGF的持续释放。目标. bFGF被认为是促进神经元损伤恢复的有效药物，然而，外源性应用bFGF由于其作用时间短而效果不佳。为了增强体内的效果，我们开发了一种新的药物输送系统，将bFGF嵌入缓慢降解的明胶水凝胶中。在这项研究中，bFGF-水凝胶对豚鼠创伤性面神经麻痹的影响进行了研究。方法.暴露颞内面神经，用显微针钳垂直夹闭。然后对动物进行以下三种程序之一：A组，不进一步处理; B组，bFGF一次性应用于神经; C组，应用bFGF-水凝胶代替。6周后，通过面部运动观察、电生理检测和组织学研究三种测试组合来评估面神经功能。结果A、B组的三项试验结果相似，提示一次性应用bFGF不利于面神经的恢复。相比之下，C组在所有测试中均取得了更好的结果。
Conclusion. Topical application of basic fibroblast growth factor (bFGF) hydrogel facilitates faster healing from traumatic facial paralysis due to continuous release of bFGF. Objectives. bFGF is considered a potent agent to facilitate recovery from neuronal damage, however, exogenously applied bFGF does not work well because of its short acting time. To enhance the effects in vivo, we developed a new drug delivery system by embedding bFGF in a gelatin hydrogel that degrades slowly. In this study, the effects of bFGF-hydrogel on traumatic facial nerve paralysis were investigated in guinea pigs. Methods. The intratemporal facial nerve was exposed and clamped at the vertical portion using micro needle forceps. The animals were then subjected to one of the following three procedures: group A, no further treatment; group B, one-shot application of bFGF to the nerve; and group C, application of bFGF-hydrogel instead. Six weeks later, facial nerve functions were evaluated by three test batteries: observation of facial movements, electrophysiological testing, and histological study. Results. The results for groups A and B were similar in the three tests, indicating that one-shot application of bFGF did not benefit facial nerve recovery. In contrast, group C achieved better results in all tests.