RESTIMULATED MEMORY TC-CELLS HAVE A HIGHER APPARENT AVIDITY OF INTERACTION WITH TARGETS THAN PRIMARY VIRUS-IMMUNE TC-CELLS AS INDICATED BY ANTI-CD8 BLOCKING
RESTIMULATED MEMORY TC-CELLS HAVE A HIGHER APPARENT AVIDITY OF INTERACTION WITH TARGETS THAN PRIMARY VIRUS-IMMUNE TC-CELLS AS INDICATED BY ANTI-CD8 BLOCKING
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DOI：
10.1038/icb.1992.33
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发表时间：
1992-08-01
影响因子：
4
通讯作者：
MULLBACHER, A
中科院分区：
文献类型：
--
作者：
HILL, AB;BLANDEN, RV;MULLBACHER, A
先前的实验表明，体外培养的二次库京免疫细胞毒性T（Tc）细胞群对感染天然黄病毒或表达免疫显性决定簇的重组痘苗病毒的靶细胞的裂解效果相同，而体内初次库京免疫Tc细胞只能裂解感染重组痘苗病毒的靶细胞。通过使用CD8阻断来评估T细胞 - 靶细胞相互作用的亲和力，感染重组病毒的靶细胞比感染天然黄病毒的靶细胞更有效地表达抗原，并且体外二次库京免疫Tc细胞对靶细胞的亲和力高于体内初次库京免疫Tc细胞。体内二次流感免疫Tc细胞对靶细胞的亲和力也高于体内初次流感免疫Tc细胞。因此，再次刺激的记忆Tc细胞群与靶细胞相互作用的亲和力比近期激活的初始细胞群更高。
Previous experiments have shown that whereas a secondary in vitro Kunjin-immune cytotoxic T (Tc) cell population lysed equally well targets infected with either native flavivirus or a recombinant vaccinia virus expressing the immunodominant determinant, primary in vivo Kunjin-immune Tc cells were able to lyse only the recombinant vaccinia virus-infected targets. Using CD8 blockade to assess the avidity of T cell-target interaction, recombinant-infected targets express antigen more efficiently than native flavivirus infected targets and secondary in vitro Kunjin-immune Tc cells have a higher avidity for targets than do primary in vivo Kunjin-immune Tc cells. Secondary in vivo influenza-immune Tc cells are also of higher avidity than primary in vivo influenza-immune Tc cells. Thus, a restimulated memory Tc cell population interacts with targets with greater avidity than does a recently activated naive population.