Noninvasive Assessment of UV-induced Skin Damage: Comparison of Optical Measurements to Histology and MMP Expression
Noninvasive Assessment of UV-induced Skin Damage: Comparison of Optical Measurements to Histology and MMP Expression
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DOI：
10.1111/j.1751-1097.2009.00634.x
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发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Uitto, Jouni
中科院分区：
文献类型：
--
作者：
Papazoglou, Elisabeth;Sunkari, Chetana;Uitto, Jouni
急性暴露于紫外线辐射（UVR）会导致可见的皮肤损伤，如红斑，并导致局部和全身免疫抑制，而慢性暴露可导致光致癌作用。这些有害作用可以通过组织学和关键生物标志物（包括基质金属蛋白酶（MMP）或色氨酸部分）的生物测定来定量。我们现在报告我们的结果在量化紫外线皮肤损伤与非侵入性光学方法的基础上反射和荧光光谱和比较这些非侵入性测量组织病理学和MMP-13的表达。研制了一种光谱输出与太阳辐射光谱输出几乎一致的太阳模拟器，并用于实验。SKH1无毛小鼠暴露于模拟太阳的紫外线辐射，总剂量为21 MED，持续10周。通过漫反射光谱法测量氧合和脱氧血红蛋白的变化，并通过荧光监测器监测色氨酸的变化。我们的研究结果表明，在无毛小鼠动物模型上，一系列皮下剂量的UV照射后，红斑、皮肤荧光、晒伤细胞和MMP-13增加。随着UV暴露的增加，观察到皮肤荧光增加。MMP-13的水平随着累积UV剂量的增加而增加，但它们的增加并不对应于非侵入性测量的变化。
Acute exposure to UV radiation (UVR) causes visible skin damage such as erythema and results in local and systemic immunosuppression while chronic exposure can result in photocarcinogenesis. These deleterious effects can be quantified by histology and by bioassays of key biological markers, including matrix metalloproteinases (MMPs), or tryptophan moieties. We now report our results in quantifying UV skin damage with noninvasive optical methods based on reflectance and fluorescence spectroscopy and compare these noninvasive measurements to histopathology and MMP-13 expression. A solar simulator with spectral output nearly identical to that of solar radiation was developed and used in our experiments. SKH1 hairless mice were exposed to solar-simulated UVR at a total dose of 21 MED delivered over 10 weeks. Changes in oxygenated and deoxygenated hemoglobin were measured by diffuse reflectance spectroscopy, and tryptophan changes were monitored via a fluorescence monitor. Our results show that there is an increase in erythema, skin fluorescence, sunburn cells and MMP-13 after a series of suberythemal doses of UV irradiation on a hairless mouse animal model. Increased skin fluorescence is observed with increasing UV exposure. The levels of MMP-13 increase as the cumulative UV dose increases but their increase does not correspond to noninvasively measured changes.