Uniform silica coated fluorescent nanoparticles: synthetic method, improved light stability and application to visualize lymph network tracer.
Uniform silica coated fluorescent nanoparticles: synthetic method, improved light stability and application to visualize lymph network tracer.
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DOI：
10.1371/journal.pone.0013167
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发表时间：
2010-10-18
期刊：
影响因子：
3.7
通讯作者：
Ohuchi N
中科院分区：
文献类型：
--
作者：
Cong L;Takeda M;Hamanaka Y;Gonda K;Watanabe M;Kumasaka M;Kobayashi Y;Kobayashi M;Ohuchi N
前哨淋巴结活检（SLNB）是近年来发展起来的一种新的淋巴结转移诊断方法。染料和放射性同位素是检测前哨淋巴结（SLN）的主要示踪剂。由于染料的尺寸较小（小于几纳米），染料往往会过度渗透到氚中，导致难以保持清晰的手术野。放射性同位素在有限的医院中可供使用。荧光纳米粒子可以选择合适的粒径和近红外荧光波长，是解决这些问题的很好的SLN示踪剂候选者。然而，纳米颗粒的使用面临安全问题，并且已经通过在纳米颗粒上提供绝缘涂层进行了许多尝试。此外，均匀绝缘层的制备对于减少作为SLN示踪剂的质量变化是重要的。本文中，我们成功地通过改进的Stöber方法在40 nm的荧光聚苯乙烯纳米颗粒上涂覆了13 nm的均匀二氧化硅层。二氧化硅包覆的纳米颗粒的光稳定性是未包覆的纳米颗粒的1.3倍。在大鼠足中注射二氧化硅涂覆的纳米颗粒可以使腘淋巴结可视化。通过透射电子显微镜可以观察到淋巴结中的二氧化硅包裹的纳米颗粒，这表明我们的二氧化硅包裹方法可用作SLN示踪剂，具有高度精确的纳米颗粒分布，用于组织学评价。我们还首次证明了前哨淋巴结的长期增强是由巨噬细胞和树突状细胞吞噬荧光纳米颗粒引起的。
The sentinel lymph node biopsy (SLNB) was developed as a new modality in the surgical diagnosis of lymph node metastases. Dye and radioisotope are major tracers for the detection of sentinel lymph nodes (SLN). Dye tends to excessively infiltrate into the interstitium due to their small size (less than several nanometers), resulting in difficulties in maintaining clear surgical fields. Radioisotopes are available in limited number of hospitals. Fluorescent nanoparticles are good candidates for SLN tracer to solve these problems, as we can choose suitable particle size and fluorescence wavelength of near-infrared. However, the use of nanoparticles faces safety issues, and many attempts have been performed by giving insulating coats on nanoparticles. In addition, the preparation of the uniform insulating layer is important to decrease variations in the quality as an SLN tracer. We herein succeeded in coating fluorescent polystyrene nanoparticles of 40 nm with uniform silica layer of 13 nm by the modified Stöber method. The light stability of silica coated nanoparticles was 1.3-fold greater than noncoated nanoparticles. The popliteal lymph node could be visualized by the silica coated nanoparticles with injection in the rat feet. The silica coated nanoparticles in lymph nodes could be observed by transmission electron microscope, suggesting that our silica coating method is useful as a SLN tracer with highly precise distribution of nanoparticles in histological evaluation. We also demonstrated for the first time that a prolonged enhancement of SLN is caused by the phagocytosis of fluorescent nanoparticles by both macrophages and dendritic cells.
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影响因子：
9.9
作者：
Kobayashi, Y;Katakami, H;Liz-Marzán, LM
通讯作者：
Liz-Marzán, LM
DOI：
10.1073/pnas.97.3.1206
发表时间：
2000-02-01
影响因子：
11.1
作者：
Yang, M;Baranov, E;Hoffman, RM
通讯作者：
Hoffman, RM
影响因子：
4.6
作者：
Soltesz, EG;Kim, S;Mihaljevic, T
通讯作者：
Mihaljevic, T
影响因子：
3.8
作者：
Keshtgar, MRS;Chicken, DW;Ell, PJ
通讯作者：
Ell, PJ
DOI：
10.2325/jbcs.14.354
发表时间：
2007-01-01
期刊：
Breast cancer (Tokyo, Japan)
影响因子：
--
作者：
Sato, Kazuhiko
通讯作者：
Sato, Kazuhiko