IMMUNOCYTOCHEMISTRY OF GABA IN THE BRAIN AND SUBESOPHAGEAL GANGLION OF MANDUCA-SEXTA
IMMUNOCYTOCHEMISTRY OF GABA IN THE BRAIN AND SUBESOPHAGEAL GANGLION OF MANDUCA-SEXTA
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DOI：
10.1007/bf01239957
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发表时间：
1987-01-01
影响因子：
3.6
通讯作者：
HILDEBRAND, JG
中科院分区：
文献类型：
--
作者：
HOMBERG, U;KINGAN, TG;HILDEBRAND, JG
用γ-氨基丁酸（GABA）特异性抗血清免疫细胞化学方法，研究了天蛾（Manducasexta）脑内和食管下神经节内GABA能神经元的分布，每个脑半球约有20000个GABA能神经元。其中大多数是视叶中间神经元，特别是形态离心神经元的板和切线神经元，支配髓质或小叶复合体。许多GABA免疫反应阳性神经元，其中巨大的纤维小叶板，项目进入正中前脑。在正中前脑的GABA免疫反应阳性神经元中，约有150个蘑菇体的pupelous负反馈纤维，支配着萼和叶，以及一组中央体下部的大扇形神经元。几个连合上和食管下神经节表现出GABA免疫反应。在食管下神经节，一组对侧下行神经元显示GABA样免疫反应。额神经节由tritcerebrum的免疫反应过程支配，但不包含GABA免疫反应胞体。除少数例外，脑神经不含GABA免疫反应纤维。
We have used specific antisera against protein-conjugatedγ-aminobutyric acid (GABA) in immunocytochemical preparations to investigate the distribution of putatively GABAergic neurons in the brain and suboesophageal ganglion of the sphinx mothManduca sexta.About 20000 neurons per brain hemisphere exhibit GABA-immunoreactivity. Most of these are optic-lobe interneurons, especially morphologically centrifugal neurons of the lamina and tangential neurons that innervate the medulla or the lobula complex. Many GABA-immunoreactive neurons, among them giant fibers of the lobula plate, project into the median protocerebrum. Among prominent GABA-immunoreactive neurons of the median protocerebrum are about 150 putatively negative-feedback fibers of the mushroom body, innervating both the calyces and lobes, and a group of large, fan-shaped neurons of the lower division of the central body. Several commissures in the supra- and suboesophageal ganglion exhibit GABA-immunoreactivity. In the suboesophageal ganglion, a group of contralaterally descending neurons shows GABA-like immunoreactivity. The frontal ganglion is innervated by immunoreactive processes from the tritocerebrum but does not contain GABA-immunoreactive somata. With few exceptions the brain nerves do not contain GABA-immunoreactive fibers.