Characterization of low molecular weight chemical fractions of dry bean (Phaseolus vulgaris) for bioactivity using Caenorhabditis elegans longevity and metabolite fingerprinting.
Characterization of low molecular weight chemical fractions of dry bean (Phaseolus vulgaris) for bioactivity using Caenorhabditis elegans longevity and metabolite fingerprinting.
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使用秀丽隐杆线虫寿命和代谢物指纹图谱来表征干豆（菜豆）的低分子量化学成分的生物活性。
DOI：
10.1021/jf1007538
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发表时间：
2010
影响因子：
6.1
通讯作者：
Thompson,HenryJ
中科院分区：
文献类型：
--
作者：
Mensack,MeghanM;Fitzgerald,VanessaK;Lewis,MatthewR;Thompson,HenryJ
据报道，食用干豆可降低患包括癌症在内的多种慢性疾病的风险。这些益处在多大程度上与植物初级代谢物和次级代谢物有关尚不清楚。本文报道的工作侧重于低分子量次级代谢物，并将野生型线虫的寿命延长作为人类健康益处的替代标记。采用改进的Bligh和Dyer技术提取冻干豆，并使用超高效液相色谱-质谱（UPLC-MS）对所得组分进行寿命延长和代谢物指纹鉴定。干豆提取物延长成人。使elego的寿命延长16%。亲水组分延长了寿命，而疏水组分则缩短了寿命。代谢物指纹图谱揭示了四个化学组分之间的显著光谱差异，并表明每个组分的化学成分在干豆遗传遗传基础上存在显著差异。
Dry bean consumption has been reported to be associated with reduced risk for a number of chronic diseases including cancer. The extent to which these benefits are associated with primary versus secondary plant metabolites is not known. The work reported herein focuses on low molecular weight secondary metabolites and uses longevity extension of wild-typeCaenorhabditiselegansnematodes as a surrogate marker for human health benefits. A modified Bligh and Dyer technique was used to extract freeze-dried bean, and the resulting fractions were evaluated for longevity extension and metabolite fingerprinting using ultra performance liquid chromatography−mass spectrometry (UPLC-MS). Dry bean extracts extended adultC. eleganslifespan by as much as 16%. Hydrophilic fractions increased lifespan, whereas the hydrophobic fraction induced longevity reduction. Metabolite fingerprinting revealed distinguishing spectral differences among the four chemical fractions evaluated and demonstrated that within each fraction chemical composition differed significantly based on dry bean genetic heritage.