Protective effect of supplemental anthocyanins on Arabidopsis leaves under high light.
Protective effect of supplemental anthocyanins on Arabidopsis leaves under high light.
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DOI：
10.1111/j.1399-3054.2009.01316.x
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发表时间：
2010-02
影响因子：
6.4
通讯作者：
Xue Zeng;W. Chow;L. Su;Xinxiang Peng;C. Peng
中科院分区：
文献类型：
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作者：
Xue Zeng;W. Chow;L. Su;Xinxiang Peng;C. Peng
从紫甘薯（Ipomoea batatas Lam.）通过高效液相色谱法结合二极管阵列检测器和电喷雾电离串联质谱法。所有花色苷都是矢车菊素或芍药苷3-槐糖苷-5-葡萄糖苷及其酰化衍生物。固相萃取法提取的紫甘薯粉中总花色苷含量为66 mg g（-1）。利用电子自旋共振技术研究了紫甘薯花色苷（PSPA）对活性氧（超氧阴离子自由基、羟自由基）和稳定的1，1-二苯基-2-苦肼基有机自由基的抑制作用。为了确定花青苷在体内叶片中的功能作用，首次将补充的花青苷渗透到花青苷生物合成缺陷的生态型兰茨贝格直立双突变体（tt 3 tt 4）的拟南芥叶片中。叶绿素荧光成像显示，花青素显着改善了光系统II在长时间的强光（1300微摩尔m（-2）s（-1））曝光的失活。DNA彗星试验表明，补充PSPA对强光诱导的叶片DNA损伤有明显的缓解作用。我们的研究结果表明，花色苷可以在体外和体内发挥作用，减轻强光胁迫对植物光合机构和DNA造成的直接或间接的氧化损伤。
Ten anthocyanin components have been detected in roots of purple sweet potato (Ipomoea batatas Lam.) by high-performance liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry. All the anthocyanins were exclusively cyanidins or peonidin 3-sophoroside-5-glucosides and their acylated derivatives. The total anthocyanin content in purple sweet potato powder obtained by solid-phase extraction was 66 mg g(-1). A strong capacity of purple sweet potato anthocyanins (PSPA) to scavenge reactive oxygen species (superoxide, hydroxyl radical) and the stable 1,1-diphenyl-2-picrylhydrazyl organic free radical was found in vitro using the electron spin resonance technique. To determine the functional roles of anthocyanins in leaves in vivo, for the first time, supplemental anthocyanins were infiltrated into leaves of Arabidopsis thaliana double mutant of the ecotype Landsberg erecta (tt3tt4) deficient in anthocyanin biosynthesis. Chlorophyll fluorescence imaging showed that anthocyanins significantly ameliorated the inactivation of photosystems II during prolonged high-light (1300 micromol m(-2) s(-1)) exposure. Comet assay of DNA revealed an obvious role of supplemental PSPA in alleviating DNA damage by high light in leaves. Our results suggest that anthocyanins could function in vitro and in vivo to alleviate the direct or indirect oxidative damage of the photosynthetic apparatus and DNA in plants caused by high-light stress.