Salt soluble without jelly-like component from the oviducal gland induces chorionic expansion in the ova of the Japanese common squid Todarodes pacificus,
Salt soluble without jelly-like component from the oviducal gland induces chorionic expansion in the ova of the Japanese common squid Todarodes pacificus,
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不含来自输卵管腺的果冻状成分的盐溶性物质可诱导日本鱿鱼 Todarodes pacificus 的卵子中绒毛膜扩张，
DOI：
10.1080/07924259.2016.1229234
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Sakurai.
中科院分区：
文献类型：
--
作者：
Adachi;K.;Yabumoto;M.;Yoo;H.;Morioka;K.;Ikeda;Y.;Ueta;Y.;Sakurai.
鱿鱼的体外受精需要雌性输卵管腺中的胶状物质；然而，这种促进受精的物质的活性成分仍然未知。在此，我们采用生化方法将太平洋Todarodes pacificus（Oegopsida）和Sepioteuthis Lessoniana（Myopsida科）的胶状物质分离成四个部分；具体来说，两种水溶性部分（分子量 > 10,000 和 < 10,000）、一种盐溶性部分和一种不溶性部分。 T 的盐溶部分。太平洋诱导的绒毛膜扩张（卵周间隙形成），先于卵子的正常胚胎发育。相反，S的盐溶部分。 Lessoniana导致卵子扩张不足，仅产生异常率较高的胚胎。这些结果表明，输卵管腺中的盐溶性成分（不是果冻状物质）诱导卵形纲的绒毛膜扩张和孵化，并且这些成分在近视纲中可能是相似的。
In vitrofertilization of squid requires the jelly substance found in the female oviducal gland; yet, the active component of this substance that facilitates fertilization remains unknown. Here, we used biochemical methods to separate the jelly substance ofTodarodes pacificus(Oegopsida) andSepioteuthis lessoniana(Myopsida family) into four fractions; specifically, two water soluble fractions (Molecular weight > 10,000 and < 10,000), one salt soluble fraction, and one insoluble fraction. The salt soluble fraction ofT. pacificusinduced chorionic expansion (perivitelline space formation), which precedes the normal embryonic development of ova. In contrast, the salt soluble fraction ofS. lessonianaelicited insufficient expansion of the ova, only producing embryos with high abnormality rates. These results suggest that the salt soluble component(s) (not the jelly-like substances) in the oviducal gland induce chorionic expansion and hatching in Oegopsida, and that these components may be similar in Myopsida.