T cell migration requires ion and water influx to regulate actin polymerization.
T cell migration requires ion and water influx to regulate actin polymerization.
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DOI：
10.1038/s41467-023-43423-8
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发表时间：
2023-12-06
影响因子：
16.6
通讯作者：
Tybulewicz, Victor L. J.
中科院分区：
文献类型：
--
作者：
de Boer, Leonard L.;Vanes, Lesley;Melgrati, Serena;O'May, Joshua Biggs;Hayward, Darryl;Driscoll, Paul C.;Day, Jason;Griffiths, Alexander;Magueta, Renata;Morrell, Alexander;MacRae, James I.;Kochl, Robert;Tybulewicz, Victor L. J.
T 细胞的迁移对于其发起免疫反应的能力至关重要。趋化因子诱导的 T 细胞迁移需要 WNK1，这是一种调节离子流入细胞的激酶。然而，尚不清楚为什么离子进入对于 T 细胞运动是必需的。在这里，我们发现来自趋化因子受体 CCR7 的信号传导导致 WNK1 及其位于迁移 CD4+ T 细胞前缘的下游通路激活，从而导致离子流入和水通过渗透进入。我们认为，WNK1 诱导的水进入需要使膜前缘膨胀，产生肌动蛋白丝可以聚合的空间，从而促进细胞向前运动。鉴于 WNK1 通路蛋白的广泛表达，我们的研究表明离子和水的流入可能对于许多细胞类型（包括白细胞和转移性肿瘤细胞）的迁移至关重要。 T 细胞的迁移能力是其功能的核心组成部分，并且已知需要 WNK1 激酶，而 WNK1 激酶与离子流入细胞有关。作者在此表明，T 细胞迁移需要 WNK1 介导的离子和水流入来膨胀前缘膜并支持肌动蛋白聚合和向前运动。
Migration of T cells is essential for their ability to mount immune responses. Chemokine-induced T cell migration requires WNK1, a kinase that regulates ion influx into the cell. However, it is not known why ion entry is necessary for T cell movement. Here we show that signaling from the chemokine receptor CCR7 leads to activation of WNK1 and its downstream pathway at the leading edge of migrating CD4+ T cells, resulting in ion influx and water entry by osmosis. We propose that WNK1-induced water entry is required to swell the membrane at the leading edge, generating space into which actin filaments can polymerize, thereby facilitating forward movement of the cell. Given the broad expression of WNK1 pathway proteins, our study suggests that ion and water influx are likely to be essential for migration in many cell types, including leukocytes and metastatic tumor cells. The ability of T cells to migrate is a central component of their functionality and is known to require WNK1 kinase that is linked to the influx of ions into the cell. Here the authors show that T cell migration requires WNK1 mediated ion and water influx to swell the membrane of the leading edge and support actin polymerisation and forward motility.
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影响因子：
2.7
作者：
Benjamini, Yoav;Krieger, Abba M.;Yekutieli, Daniel
通讯作者：
Yekutieli, Daniel
影响因子：
8.8
作者：
Ayasoufi, Katayoun;Kohei, Naoki;Valujskikh, Anna
通讯作者：
Valujskikh, Anna
影响因子：
3.4
作者：
Clausen, M. P.;Colin-York, H.;Fritzsche, M.
通讯作者：
Fritzsche, M.
影响因子：
5.7
作者：
通讯作者：
--
DOI：
10.1126/science.aay7794
发表时间：
2020-06-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bisaria A;Hayer A;Garbett D;Cohen D;Meyer T
通讯作者：
Meyer T