Acute lung injury induced by intestinal ischemia and reperfusion is altered in obese female mice
Acute lung injury induced by intestinal ischemia and reperfusion is altered in obese female mice
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DOI：
10.1016/j.pupt.2018.01.005
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发表时间：
2018-04-01
影响因子：
3.2
通讯作者：
Riffo-Vasquez, Yanira
中科院分区：
文献类型：
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作者：
Fantozzi, Evelyn Thais;Rodrigues-Garbin, Sara;Riffo-Vasquez, Yanira
理由：急性肺损伤（acute lung injury，ALI）是肠缺血再灌注（intestinal ischemia and reperfusion，I/R）损伤后常见的并发症，可导致急性呼吸窘迫综合征（acute respiratory distress syndrome，ARDS）。我们以前已经证明，女性是保护免受肠I/R诱导的肺损伤通过雌激素介导mechanism.Objectives：探讨肥胖对急性肺损伤诱导肠I/Rin female mice. Methods：C57 B1/6雌性小鼠喂养标准的低脂肪饮食（SD）或高脂肪饮食（HFD）9周。结果：SD和HFD小鼠在再灌注2 h和24 h时，肺髓过氧化物酶（MPO）表达和中性粒细胞数均显著增加。此外，与SD小鼠相比，HFD小鼠的肺嗜酸性粒细胞过氧化物酶（EPO）表达和嗜酸性粒细胞数量显著增加。与SD小鼠相比，HFD小鼠在再灌注2 h和24 h时肺湿/干重比显著增大，同时肺组织中诱导型NO表达显著增加，动脉血氧饱和度显著降低。肥胖易使雌性小鼠肺水肿增加和气体交换恶化，这伴随着肺中iNOS表达的增加。
Rational: Acute lung injury (ALI) is a common complication after intestinal ischemia and reperfusion (I/R) injury that can lead to acute respiratory distress syndrome (ARDS). We have previously demonstrated that females are protected against lung damage induced by intestinal I/R through an estrogen mediated mechanism.Objectives: To investigate the effect of obesity on ALI induced by intestinal I/R in female mice.Methods: C57B1/6 female mice were fed with a standard low-fat diet (SD) or a high-fat diet (HFD) for 9 weeks. Intestinal I/R injury was induced by a 45 min occlusion of the mesenteric artery followed by 2 and 24 h of reperfusion.Results: Significant increase in lung myeloperoxidase expression (MPO) and neutrophil numbers of SD and HFD mice occurred at 2 h and 24 h of reperfusion. Furthermore, HFD mice presented a significant increase in lung eosinophil peroxidase (EPO) expression and eosinophil numbers compared to SD mice. Lung wet/dry weight ratio was significantly greater in HFD mice at 2 and 24 h of reperfusion, accompanied by a significant increase in the expression of inducible NO in the lung tissue and a significant decrease in arterial oxygen saturation at 24 h of reperfusion relative to SD mice.Conclusion: Obesity predisposes female mice to increased pulmonary oedema and deterioration in gas exchange, which is accompanied by an increase in iNOS expression in the lung.