Enhanced radiosensitivity by inhibition of nuclear factor kappa B activation in human malignant glioma cells
Enhanced radiosensitivity by inhibition of nuclear factor kappa B activation in human malignant glioma cells
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DOI：
10.1080/095530097143374
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发表时间：
1997-08-01
影响因子：
2.6
通讯作者：
Takebe, H
中科院分区：
文献类型：
--
作者：
Yamagishi, N;Miyakoshi, J;Takebe, H
为明确细胞放射敏感性与核因子κ B （nf - κ B）活化的关系，构建nf - κ B细胞抑制蛋白I κ B- α表达质粒，转染2株人恶性胶质瘤细胞株。过表达I κ pa b - α蛋白的细胞比亲本细胞和一个低表达的转染克隆具有更高的辐射敏感性。在亲本细胞系和一个低表达的转染克隆中，NF-kappa B的序列特异性dna结合活性在辐照后1 ~ 2 h内显著提高。相比之下，转染过表达I κ pa b - α蛋白的克隆的dna结合活性没有增加。这些结果提示nf - κ B的活化可能是决定细胞放射敏感性的内在反应之一。
To clarify the relationship between cellular radiosensitivity and nuclear factor kappa B (NF-kappa B) activation, an expression plasmid was constructed for I kappa B-alpha, a cellular inhibitory protein of NF-kappa B, and transfected it into two human malignant glioma cell lines. Cells overexpressing the I kappa B-alpha protein were more radiosensitive than the parental cells and one transfected clone with low expression. In the parental cell lines and one transfected clone with low expression, the sequence specific DNA-binding activity of NF-kappa B was considerably increased between 1 and 2 h after irradiation. In contrast, no increase in the DNA-binding activity was observed in the transfected clone overexpressing I kappa B-alpha protein. These results suggest that the activation of NF-kappa B may be one of the intrinsic responses determining cellular radiosensitivity.