VOLUME CONTROL OF ERYTHROCYTES DURING STORAGE - THE ROLE OF MANNITOL
VOLUME CONTROL OF ERYTHROCYTES DURING STORAGE - THE ROLE OF MANNITOL
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DOI：
10.1046/j.1537-2995.1988.28488265266.x
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发表时间：
1988-07-01
期刊：
影响因子：
2.9
通讯作者：
KUHL, W
中科院分区：
文献类型：
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作者：
BEUTLER, E;KUHL, W
当红细胞作为悬浮液储存在人工防腐剂溶液中时，例如由盐水、腺嘌呤和葡萄糖组成的溶液，在体外发生红细胞溶血增加，这种现象很容易通过添加甘露醇来防止。甘露醇防止溶血的机制尚不清楚。作者研究了甘露醇通过防止红细胞的渗透性肿胀来防止溶血的可能性，否则红细胞的体积可能会增加到超过其临界溶血体积。储存在人工培养基中确实会导致红细胞肿胀，甘露醇可以防止红细胞肿胀，加入制霉菌素可以增加红细胞肿胀，制霉菌素是一种能大大增加红细胞钠-钾渗透性的药物。在不同浓度的甘露醇下，肿胀和溶血是相关的，但当考虑到制霉菌素处理的细胞时，这种相关性就消失了;即使有明显的肿胀，溶血也相对较少。此外，通过渗透手段急剧增加储存的红细胞的体积导致相对较少的额外溶血。这些发现表明，单纯的渗透溶解不太可能是储存细胞溶血的原因。甘露醇的其他一些稳定作用可能与渗透作用有关，也可能无关，但肯定也起了一定的作用。
When red cells are stored as suspensions in artificial preservative solutions such as those consisting of saline, adenine, and glucose, increased hemolysis of the red cells occurs in vitro, a phenomenon that is readily prevented by the addition of mannitol. The mechanism by which mannitol prevents hemolysis is unknown. The authors have examined the possibility that mannitol prevents hemolysis by prevention of osmotic swelling of red cells that might otherwise increase their volume beyond their critical hemolytic volume. Storage in artificial media does, indeed, result in swelling of the red cells, which is prevented by mannitol and is increased by the addition of nystatin, an agent that greatly increases the sodium-potassium permeability of the cells. Swelling and hemolysis are correlated at different concentrations of mannitol, but the correlation breaks down when the case of nystatin-treated cells is considered; even with marked swelling, there is relatively little hemolysis. Moreover, increasing the volume of the stored red cells acutely by osmotic means results in relatively little additional hemolysis. These findings make it appear unlikely that simple osmotic lysis is the cause of hemolysis in stored cells. Some other stabilizing effect of mannitol that may or may not be related to its osmotic effect must play a role.