Human peripheral blood hematopoietic progenitors are optimal targets of retroviral-mediated gene transfer.
Human peripheral blood hematopoietic progenitors are optimal targets of retroviral-mediated gene transfer.
复制标题
人外周血造血祖细胞是逆转录病毒介导的基因转移的最佳靶标。
作者：
M. Bregni;M. Magni;Salvatore Siena;M. Nicola;G. Bonadonna;A. M. Gianni
Hematopoietic progenitor cells circulate in the peripheral blood (PB) of cancer patients during the recovery phase that follows treatment with high-dose cyclophosphamide followed by hematopoietic growth factor infusion. We report that when PB progenitors were exposed in vitro to filtered supernatant from cell line PA317-N2, producing amphotropic helper-free N2 vector at conventional titers, successful retroviral-mediated transfer of neomycin resistance gene was documented by polymerase chain reaction in 93% of day 14 myelomonocytic colonies. Under the same conditions, gene transfer was achieved in 22% of steady-state bone marrow-derived myelomonocytic colonies. Neo-resistance gene transfer was documented also in a CD34+/cyclophosphamide-resistant precursor to granulocyte-macrophage colonies, an undifferentiated progenitor close to the hematopoietic stem cell. Neither cocultivation with vector-producing cells nor high vector titer were stringent requisites for efficient gene transfer. The large-scale availability of PB hematopoietic progenitors in cancer patients, together with the high gene transfer rate achieved under safe and clinically feasible conditions, support an optimal approach for gene transfer procedures into the human hematopoietic system.
逆转录病毒介导的 NEOr 基因转移后，保护长期培养中未感染的人骨髓细胞免受 G418 毒性。
影响因子：
3.7
作者：
Bayever,E;Haines,K;Duprey,S;Rappaport,E;Douglas,SD;Surrey,S
通讯作者：
Surrey,S
人类造血前体群体的纯化和部分表征。
影响因子：
20.3
作者：
Smith,C;Gasparetto,C;Collins,N;Gillio,A;Muench,MO;O'Reilly,RJ;Moore,MA
通讯作者：
Moore,MA
将腺苷脱氨酶基因转移到原始人类造血祖细胞中。
影响因子：
4.2
作者：
Cournoyer,D;Scarpa,M;Mitani,K;Moore,KA;Markowitz,D;Bank,A;Belmont,JW;Caskey,CT
通讯作者：
Caskey,CT