Monitoring of human cytomegalovirus infections in pediatric bone marrow transplant recipients by nucleic acid sequence-based amplification.
Monitoring of human cytomegalovirus infections in pediatric bone marrow transplant recipients by nucleic acid sequence-based amplification.
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通过基于核酸序列的扩增监测儿科骨髓移植受者的人类巨细胞病毒感染。
DOI：
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发表时间：
1998
影响因子：
6.4
通讯作者：
Koichi Yamanishi
中科院分区：
文献类型：
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作者：
T. Aono;Kazuhiro Kondo;H. Miyoshi;K. Tanaka;Motohiro Kondo;Y. Osugi;Junichi Hara;S. Okada;Koichi Yamanishi
在人巨细胞病毒（HCMV）感染的诊断中，区分有症状和无症状感染是非常重要的。将基于核酸序列的扩增（NASBA）技术与单一和巢式聚合酶链反应（PCR）方法进行了比较。对于NASBA检测，选择β 2.7转录物作为靶标，因为其丰富的活性HCMV特异性表达。在20例儿童骨髓移植（BMT）受者中，8例出现HCMV相关临床症状。单PCR的敏感性和特异性分别为50%和100%，巢式PCR为100%和67%，NASBA为100%和83%。对儿童BMT受者中HCMV感染的随访表明，NASBA既可以在临床症状发作之前检测病毒转录物，又可以反映抗病毒治疗引起的临床改善。这些数据表明，NASBA应该是有用的预测HCMV疾病的发展和监测抗病毒治疗的效果。
In the diagnosis of human cytomegalovirus (HCMV) infection, it is very important to distinguish symptomatic from asymptomatic infection. The nucleic acid sequence-based amplification (NASBA) technique was compared with single and nested polymerase chainreaction (PCR) methods. For NASBA detection, the beta2.7 transcript was chosen as a target because of its abundant active HCMV-specific expression. Of 20 pediatric bone marrow transplant (BMT) recipients, 8 developed HCMV-related clinical symptoms. The clinical sensitivities and specificities were 50% and 100% for single PCR, 100% and 67% for nested PCR, and 100% and 83% for NASBA, respectively. Follow-up of HCMV infections in pediatric BMT recipients showed that NASBA could both detect viral transcript prior to the onset of clinical symptoms and reflect clinical improvement due to antiviral therapy. These data suggest that NASBA should be useful for both predicting HCMV disease development and monitoring the effect of antiviral therapy.
利用尿液和白细胞培养以及血浆 DNA 聚合酶链式反应来识别有患人类巨细胞病毒疾病风险的艾滋病患者。
DOI：
10.1093/infdis/175.2.302
发表时间：
1997
期刊：
The Journal of infectious diseases
影响因子：
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作者：
Shinkai,M;Bozzette,SA;Powderly,W;Frame,P;Spector,SA
通讯作者：
Spector,SA