A Novel Type of Dual-Modality Molecular Probe for MR and Nuclear Imaging of Tumor: Preparation, Characterization and in Vivo Application
A Novel Type of Dual-Modality Molecular Probe for MR and Nuclear Imaging of Tumor: Preparation, Characterization and in Vivo Application
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一种用于肿瘤 MR 和核成像的新型双模态分子探针：制备、表征和体内应用
DOI：
10.1021/mp900143a
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发表时间：
2009-07-01
影响因子：
4.9
通讯作者：
Gao, Mingyuan
中科院分区：
文献类型：
--
作者：
Liu, Shujie;Jia, Bing;Gao, Mingyuan
设计并制备了一种由生物相容性良好的Fe 3 O 4纳米粒子、单克隆抗体和放射性核素组成的新型双模态分子探针。双模态分子探针中的所有功能组分，Fe 3 O 4、PEG、mAb 3 H11和12 I-125通过化学键合形成稳定的分子探针。进行系统的体外实验以评价靶向探针中抗体的生物活性。基于该双模态成像探针，通过MRI和γ成像技术检测裸鼠移植瘤，进行了一系列体内实验。研究了双模态分子探针在荷瘤裸鼠体内的药代动力学。
A novel dual-modality molecular probe composed of biocompatible Fe3O4 nanocrystal, monoclonal antibody and radionuclide was designed and prepared. All functional components in the dual-modality molecular probe, i.e., Fe3O4, PEG, mAb 3H11 and 12 I-125, were chemically bonded together for forming a stable molecular probe. Systematic in vitro experiments were carried out for evaluating the biological activity of the antibody in the targeting probe. A series of in vivo experiments were performed based on the dual-modality imaging probe for detecting xenografted tumors in nude mice by MRI and gamma-imaging techniques. The pharmacokinetics of the dual-modality molecular probe in tumor-bearing nude mice was studied.