Aggregation-Induced Emission Luminogen-Based Dual-Mode Enzyme-Linked Immunosorbent Assay for Ultrasensitive Detection of Cancer Biomarkers in a Broad Concentration Range
Aggregation-Induced Emission Luminogen-Based Dual-Mode Enzyme-Linked Immunosorbent Assay for Ultrasensitive Detection of Cancer Biomarkers in a Broad Concentration Range
复制标题
基于聚集诱导发射发光原的双模式酶联免疫吸附测定，用于宽浓度范围内癌症生物标志物的超灵敏检测
DOI：
10.1021/acssensors.1c02237
复制
发表时间：
2022
期刊：
影响因子：
8.9
通讯作者：
Tang Beng Zhong
中科院分区：
文献类型：
--
作者：
Shen Jianlei;Situ Bo;Du Xianchao;Wang Zhiming;Hu Rong;Li Baixue;Qin Anjun;Tang Beng Zhong
酶联免疫吸附试验（ELISA）是测定生物样品中抗体和抗原的最常用方法之一。然而，开发具有高检测灵敏度和宽检测动态范围的新ELISA，而不诉诸复杂的信号处理和设备设置仍然是一个挑战。在这项工作中，我们报告了一种策略，同时提高检测灵敏度和拓宽的动态范围，取代显色试剂在传统的ELISA与聚集诱导发射发光团（AIEgen）。所建立的AIE-ELISA可产生互补的吸光度和荧光信号，线性检测范围为1.6- 25，000 pg/mL。该双模式AIE-ELISA在前列腺特异性抗原（PSA）检测中的应用实现了1.3pg/mL（3.78 × 10- 14 M）的检测限和与单模式ELISA相比约2个数量级的动态范围改善，这使得其能够区分患者血清中的微小PSA差异。与传统的ELISA显色试剂相比，AIEgen具有操作简单、酶反应速度快、光稳定性好等优点，在免疫分析、免疫组织化学和免疫细胞化学等领域具有广阔的应用前景。
The enzyme-linked immunosorbent assay (ELISA) is one of the most commonly used methods for measuring antibodies and antigens in biological samples. However, developing new ELISAs with high detection sensitivity and broad detection dynamic ranges without resorting to complicated signal processing and equipment setups remains a challenge. In this work, we report a strategy to simultaneously improve the detection sensitivity and broaden the dynamic range by replacing the chromogenic reagents used in traditional ELISAs with an aggregation-induced emission luminogen (AIEgen). The developed AIE–ELISA could generate complementary absorbance and fluorescence signals with a linear detection range of 1.6–25,000 pg/mL. The application of this dual-mode AIE–ELISA in the detection of the prostate-specific antigen (PSA) realized a limit of detection of 1.3 pg/mL (3.78 × 10–14M) and dynamic range improvement of approximately 2 orders of magnitude compared to a single-mode ELISA, which enabled it to discriminate a minor PSA difference in a patient’s serum. The simpler experimental operation, faster enzyme response speed, and better photostability of AIEgen than the traditional chromogenic reagents used in ELISAs showed that our developed AIE–ELISA holds great potential in the fields of immunoassay, immunohistochemistry, and immunocytochemistry.