Association of Gut Microbiota during Early Pregnancy with Risk of Incident Gestational Diabetes Mellitus
Association of Gut Microbiota during Early Pregnancy with Risk of Incident Gestational Diabetes Mellitus
复制标题
妊娠早期肠道微生物群与妊娠期糖尿病风险的关联。
DOI：
10.1210/clinem/dgab346
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发表时间：
2021-10-01
影响因子：
5.8
通讯作者：
Pan, An
中科院分区：
文献类型：
--
作者：
Hu, Ping;Chen, Xiuyi;Pan, An
目的：我们旨在评估中国孕妇妊娠早期肠道细菌生物标志物与妊娠期糖尿病 (GDM) 风险之间的关联。方法：在同济-双流出生队列研究中，我们对 201 例 GDM 病例和 201 例匹配对照进行了巢式病例对照研究。在怀孕早期（6-15周）收集粪便样本，并在怀孕24至28周时诊断出GDM。对从粪便样本中分离的群落 DNA 和 16S rRNA 基因扩增子文库的 V3-V4 区域进行了测序。结果：在 GDM 病例与对照中，Rothia、放线菌、双歧杆菌、Adlercreutzia、Coriobacteriaceae 和 Lachnospiraceae spp。显着减少，而肠杆菌科、瘤胃球菌科和韦荣球菌科的比例过高。此外，作为参考微生物，葡萄球菌相对于梭状芽胞杆菌、罗斯布里亚菌和红曲菌科的丰度与空腹血糖、餐后1小时和2小时血糖水平呈正相关。将微生物分类群添加到具有常规风险因素的基本 GDM 预测模型中，C 统计量显着增加 (P < 0.001)，从 0.69 增加到 0.75。结论：妊娠早期肠道微生物群与随后的 GDM 风险相关。一些有益和共生的肠道微生物与 GDM 的发生呈负相关，而机会致病菌则与 GDM 的较高风险相关，并与 OGTT 的血糖水平呈正相关。
Aims: We aimed to assess the association between gut bacterial biomarkers during early pregnancy and subsequent risk of gestational diabetes mellitus (GDM) in Chinese pregnant women.Methods: Within the Tongji-Shuangliu Birth Cohort study, we conducted a nested case-control study among 201 incident GDM cases and 201 matched controls. Fecal samples were collected during early pregnancy (at 6-15 weeks), and GDM was diagnosed at 24 to 28 weeks of pregnancy. Community DNA isolated from fecal samples and V3-V4 region of 16S rRNA gene amplicon libraries were sequenced.Results: In GDM cases versus controls, Rothia, Actinomyces, Bifidobacterium, Adlercreutzia, and Coriobacteriaceae and Lachnospiraceae spp. were significantly reduced, while Enterobacteriaceae, Ruminococcaceae spp., and Veillonellaceae were overrepresented. In addition, the abundance of Staphylococcus relative to Clostridium, Roseburia, and Coriobacteriaceae as reference microorganisms were positively correlated with fasting blood glucose, 1-hour and 2-hour postprandial glucose levels. Adding microbial taxa to the base GDM prediction model with conventional risk factors increased the C-statistic significantly (P < 0.001) from 0.69 to 0.75.Conclusions: Gut microbiota during early pregnancy was associated with subsequent risk of GDM. Several beneficial and commensal gut microorganisms showed inverse relations with incident GDM, while opportunistic pathogenic members were related to higher risk of incident GDM and positively correlated with glucose levels on OGTT.