DNA Adsorption to Laterally Structured Charged Lipid Membranes
DNA Adsorption to Laterally Structured Charged Lipid Membranes
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DOI：
10.1021/la990273b
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发表时间：
1999-10
期刊：
影响因子：
3.9
通讯作者：
H. Clausen‐Schaumann;H. Gaub
中科院分区：
文献类型：
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作者：
H. Clausen‐Schaumann;H. Gaub
宏观表面特性，例如表面对某种聚合物的亲和力，可能很大程度上取决于纳米尺度长度尺度的表面参数，例如具有正表面电荷和负表面电荷的区域的大小。在这项研究中，研究了聚电解质对横向结构带电表面的吸附与带电表面积大小的函数关系。聚脱氧核糖核酸（DNA）被吸附到嵌入负表面电位基质中的支撑阳离子脂质双层上，并且阳离子表面积的大小从微米到亚纳米长度尺度变化。用原子力显微镜对支撑的脂质双层和吸附的 DNA 进行成像，发现 DNA 吸附取决于表面电荷密度和阳离子脂质双层区域的大小。虽然在大于 20 nm 的纯阳离子脂质双层上可以观察到密集的 DNA 层，但正如预期的那样，在 m...
A macroscopic surface property, such as a surface's affinity for a certain polymer, may depend significantly on surface parameters on the nanoscopic length scale, such as the size of areas with positive and negative surface charge. In this study, polyelectrolyte adsorption to laterally structured charged surfaces was investigated as a function of the size of the charged surface areas. Polydeoxyribonucleic acid (DNA) was adsorbed onto supported cationic lipid bilayers which were imbedded in a matrix of negative surface potential, and the size of the cationic surface areas was varied from the micrometer down to the sub-nanometer length scale. The supported lipid bilayers and the adsorbed DNA were imaged with an atomic force microscope, and the DNA adsorption was found to depend on the surface charge density and on the size of the cationic lipid bilayers areas. While on purely cationic lipid bilayers larger than 20 nm a densely packed layer of DNA could be observed, as expected, no DNA could be detected on m...