Intake of Tomato-Enriched Diet Protects from 6-Hydroxydopamine-Induced Degeneration of Rat Nigral Dopaminergic Neurons
Intake of Tomato-Enriched Diet Protects from 6-Hydroxydopamine-Induced Degeneration of Rat Nigral Dopaminergic Neurons
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DOI：
10.1007/978-3-211-92660-4_28
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Esposito, Ennio
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文献类型：
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作者：
di Matteo, Vincenzo;Pierucci, Massimo;Esposito, Ennio
大量证据表明，黑质致密部 (SNc) 中含有多巴胺 (DA) 的神经元的氧化损伤可能与帕金森病 (PD) 的发病机制有关。我们在 PD 动物模型中评估了富含野生型红塞特 (RS) 番茄或富含 β-胡萝卜素（通过激活番茄红素 β-环化酶 (tlcy-b) 获得的转基因高胡萝卜素 (HC) 番茄）的饮食的潜在神经保护作用。雄性 Fischer 344 大鼠用标准 Altromin(MT) 饮食、5% RS 或 5% HC 富集饮食喂养 14 天。开始这种饮食方案 7 天后，将 6-羟基多巴胺 (6-OHDA) 注射到左侧 SNc 中，使大鼠产生损伤。再过7天后，处死大鼠，测量左侧（同侧）和右侧（对侧）纹状体中的DA和3,4-二羟基苯乙酸（DOPAC）水平。对照组纹状体 DA 水平降低了 86.5 +/- 5.0%，HC 组降低了 86.2 +/- 5.0%，RS 喂养组降低了 56.0 +/- 9.0%。对照组纹状体 DOPAC 降低了 85.6 +/- 5.0%，HC 组降低了 83.0 +/- 6.0%，RS 喂养组降低了 58.9 +/- 10.0%。第14天从大鼠中采集血液，并通过液相色谱-大气压化学电离-质谱法(LC-APCI-MS)测量番茄红素和β-胡萝卜素的血浆水平，以测定番茄红素和β-胡萝卜素的水平。 5% RS 喂养的大鼠中番茄红素的血浆水平为 4.7 +/- 0.2 ng/ml，而对照和 HC 喂养的大鼠中检测不到（< 2.5 ng ml(-1)）。 RS 饮食对保护纹状体多巴胺能神经支配的功效可归因于番茄红素能够防止 SNc 中含有 DA 的神经元变性。
There is extensive evidence that oxidative damage of dopamine (DA)-containing neurons in the substantia nigra pars compacta (SNc) may contribute to the pathogenesis of Parkinson's disease (PD). We evaluated the potential neuroprotective effect of diets enriched with wild-type Red Setter (RS) tomato or transgenic High Carotene (HC) tomato, rich in beta-carotene, obtained by the activation of lycopene beta-cyclase (tlcy-b), in an animal model of PD. Male Fischer 344 rats were fed for 14 days with standard Altromin(MT) diet, 5% RS- or 5% HC-enriched diet. Seven days after the beginning of this diet regimen, the rats were lesioned by 6-hydroxydopamine (6-OHDA) injected into the left SNc. After further 7 days, the rats were sacrificed, and DA and 3,4-dihydroxyphenylacetic acid (DOPAC) levels in both the left (ipsilateral) and the right (contralateral) striata were measured. Striatal DA levels were reduced by 86.5 +/- 5.0% in control, 86.2 +/- 5.0% in HC-, and 56.0 +/- 9.0% in RS- fed group. Striatal DOPAC was decreased by 85.6 +/- 5.0% in controls, 83.0 +/- 6.0% in HC-, and 58.9 +/- 10.0% in RS- fed group. Blood was obtained from the rats on day 14 and the plasma level of licopene and beta-carotene was measured by liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS) for the determination of lycopene and beta-carotene levels. The plasma level of lycopene was 4.7 +/- 0.2 ng/ml in 5% RS- fed rats, while it was undetectable (< 2.5 ng ml(-1)) in control and HC- fed rats. The efficacy of RS diet to preserve striatal dopaminergic innervation can be attributed to the ability of lycopene to prevent the degeneration of DA-containing neurons in the SNc.