Growth performance and protective effect of vitamin E on oxidative stress pufferfish (Takifugu obscurus) following by ammonia stress
Growth performance and protective effect of vitamin E on oxidative stress pufferfish (Takifugu obscurus) following by ammonia stress
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DOI：
10.1007/s10695-018-0468-2
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发表时间：
2018-04-01
影响因子：
2.9
通讯作者：
Wang, An-Li
中科院分区：
文献类型：
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作者：
Cheng, Chang-Hong;Guo, Zhi-Xun;Wang, An-Li
本试验旨在研究维生素E对氨胁迫下河豚生长性能、生化指标和抗氧化能力的影响。分别以2.31（对照）、21.84、40.23、83.64、158.93和311.64 mg kg（-1）干重添加维生素E的试验基础饲料饲喂60 d。饲喂试验结束后，在100 mg L-1氨氮环境下处理48 h。结果表明，维生素E组显著提高了鱼的增重、特定生长率以及生长激素受体和胰岛素样生长因子的表达水平。饲粮中添加维生素e可提高血浆碱性磷酸酶活性，降低血浆谷草转氨酶和谷丙转氨酶活性。热休克蛋白（40.23 ~ 311.64 mg kg（-1）维生素E饲粮组）、锰超氧化物歧化酶（83.64 ~ 158.93 mg kg（-1）维生素E饲粮组）、过氧化氢酶（40.23 ~ 311.64 mg kg（-1）维生素E饲粮组）和谷胱甘肽还原酶（40.23 ~ 311.64 mg kg（-1）维生素E饲粮组）相对表达量上调。另一方面，83.64 ~ 311.64 mg kg（-1）维生素E添加组活性氧（ROS）水平显著降低。这些结果表明，维生素E作为一种有效的抗氧化剂可能具有潜在的有用作用，可以提高河豚的抵抗力。
This study was conducted to determine the effects of vitamin E on growth performance, biochemical parameters, and antioxidant capacity of pufferfish (Takifugu obscurus) exposed to ammonia stress. The experimental basal diets supplemented with vitamin E at the rates of 2.31 (control), 21.84, 40.23, 83.64, 158.93, and 311.64 mg kg(-1) dry weight were fed to fish for 60 days. After the feeding trial, the fish were exposed to 100 mg L-1 ammonia-nitrogen for 48 h. The results shown that the vitamin E group significantly improved weight gain, specific growth rate, and the expression levels of growth hormone receptors and insulin-like growth factor. Fish fed with the vitamin E-supplemented diets could increase plasma alkaline phosphatase activities and decrease plasma glutamicoxalacetic transaminase and glutamic-pyruvic transaminase activities. The relative expression levels of heat shock proteins (40.23-311.64 mg kg(-1) vitamin E diet group), manganese superoxide dismutase (83.64-158.93 mg kg(-1) vitamin E diet group), catalase (40.23-311.64 mg kg(-1) vitamin E diet group), and glutathione reductase (40.23-311.64 mg kg(-1) vitamin E diet group) were upregulated. On the other hand, the decreased level of reactive oxygen species (ROS) was observed in the 83.64-311.64 mg kg(-1) vitamin E additive group. These results showed that vitamin E might have a potentially useful role as an effective antioxidant to improve resistance in pufferfish.