Early development of the Japanese spiny oyster (Saccostrea kegaki):: Characterization of some genetic markers

Early development of the Japanese spiny oyster (Saccostrea kegaki):: Characterization of some genetic markers
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DOI:
10.2108/zsj.25.455
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发表时间:
2008-05-01
期刊:
影响因子:
0.9
通讯作者:
Wada, Hiroshi
Wada, Hiroshi
中科院分区:
生物学4区
文献类型:
--
作者:
Kakoi, Shota;Kin, Koryu;Wada, Hiroshi

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软体动物门是散轮动物门的主要类群之一。虽然软体动物表现出很大的形态多样性,只有少数比较胚胎学研究已经进行了这一组。在本研究中,开始了解分子发育的不同形态之间的软体动物,我们观察了早期胚胎发生的双壳类,日本棘牡蛎,囊牡蛎kegaki。虽然一些研究已经开始揭示腹足类早期发育的遗传机制,但关于双壳类胚胎发生的分子信息很少。因此,作为确定组织特异性基因标记的一步,我们对从原肠胚阶段cDNA文库中随机挑选的约100个cDNA克隆进行了测序。这些基本的胚胎学信息将有助于进一步研究软体动物的发育和进化。
The phylum Mollusca is one of the major groups of Lophotrochozoa. Although mollusks exhibit great morphological diversity, only a few comparative embryological studies have been performed on this group. In the present study, to begin understanding the molecular development of the diverse morphology among mollusks, we observed early embryogenesis in a bivalve, the Japanese spiny oyster, Saccostrea kegaki. Although several studies have begun to reveal the genetic machinery for early development in gastropods, very little molecular information is available on bivalve embryogenesis. Thus, as a step toward identifying tissue-specific gene markers, we sequenced about 100 cDNA clones picked randomly from a gastrula-stage cDNA library. This basic information on bivalve embryology will be useful for further studies on the development and evolution of mollusks.