An efficient protocol for inducing pseudopregnancy using estradiol dipropionate and follicular development associated with changes in reproductive hormones after prostaglandin F2alpha treatment in pseudopregnant sows
An efficient protocol for inducing pseudopregnancy using estradiol dipropionate and follicular development associated with changes in reproductive hormones after prostaglandin F2alpha treatment in pseudopregnant sows
复制标题
DOI：
10.1186/1477-7827-9-157
复制
发表时间：
2011-12-14
影响因子：
4.4
通讯作者：
Kaneko, Hiroyuki
中科院分区：
文献类型：
--
作者：
Noguchi, Michiko;Yoshioka, Koji;Kaneko, Hiroyuki
背景：同期发情技术在畜牧业中的应用将为畜群的繁殖管理提供更多的选择。为了探索一种简便的猪同期发情方法，本研究确定了用二丙酸雌二醇（EDP）建立猪同期发情模型的有效方案，并研究了前列腺素F2 α（PGF 2 α）对猪同期发情过程中卵泡发育的影响（PGF 2 α）与假孕母猪生殖激素谱的关系。在实验1中，研究了在周期性猪中单次施用EDP以诱导假孕的有效剂量（0、10、20或30 mg）和时间（排卵后5、8、11或13天）。在实验2中，4头假孕母猪在EDP给药后24 - 28天之间以24小时间隔接受PGF 2 α给药两次。用时间分辨荧光免疫分析法分析血浆生殖激素浓度的变化。卵泡发育和排卵PGF 2 α管理后监测transrectal ultrasonography.Results：高效率（大于80%）的pseudopregulatory实现了一个单一的治疗与20毫克的EDP在排卵后8和11天（相当于9-13天后开始发情）。假孕母猪血浆雌二醇-17 β浓度在12小时和7天之间显著高于EDP治疗前。EDP处理后，总甜菜碱浓度显着下降，并保持低水平14天。小卵泡数量从PGF 2 α处理的6.3 +/- 2.6增加至3天后的22.8 +/- 4.8;这与Escherichin血浆浓度增加相关。PGF 2 α治疗后5.3 +/- 0.3天，所有母猪均可检测到动情期开始，治疗后7.6 +/- 0.2天检测到排卵卵泡数为15.5 +/- 1.4。结论：本研究确定了诱导周期猪假孕的EDP治疗的有效剂量和时间。我们的研究结果还表明，EDP引起的降低，在pseudopregulatory和少量的卵泡，从20至28天后，EDP的卵泡素浓度。相比之下，EDP诱导的假性孕激素似乎对卵泡发育和随后的PGF 2 α给药后排卵无不良影响。
Background: Utilization of estrus synchronization program in livestock industry would provide greater options for reproductive management in herd. To develop a convenient method for estrus synchronization in pigs, we determined the effective protocol using estradiol dipropionate (EDP) for the establishment of pseudopregnancy and investigated follicular development during the estrus synchronization with prostaglandin F2alpha (PGF2alpha) in association with reproductive hormone profiles in pseudopregnant sows.Methods: In Experiment 1, the effective dose (0, 10, 20, or 30 mg) and timing (5, 8, 11 or 13 days after ovulation) of a single administration of EDP in cyclic pigs for the induction of pseudopregnancy were investigated. In Experiment 2, four pseudopregnant sows were treated with PGF2alpha twice at a 24-h interval between 24 and 28 days after EDP treatment. The changes in plasma concentrations of reproductive hormones were analyzed by time-resolved fluoroimmunoassay. Follicular development and ovulation following PGF2alpha administration were monitored by transrectal ultrasonography.Results: High efficiency (greater than 80%) of pseudopregnancy was achieved with a single treatment with 20 mg of EDP at 8 and 11 days after ovulation (equivalent to 9-13 days after the onset of estrus). Plasma estradiol-17beta concentrations in pseudopregnant sows were significantly higher between 12 h and 7 days than before EDP treatment. Total inhibin concentrations significantly decreased following EDP treatment and remained low for 14 days. The number of small follicles was increased from 6.3 +/- 2.6 at PGF2alpha treatment to 22.8 +/- 4.8 at 3 days later; this was associated with increased plasma concentrations of inhibin. Onset of estrus was detectable in all sows on 5.3 +/- 0.3 days after PGF2alpha treatment and the number of ovulated follicles was 15.5 +/- 1.4 detected at 7.6 +/- 0.2 days after the treatment.Conclusions: This study has defined the effective dose and timing of EDP treatment for inducing pseudopregnancy in cyclic pigs. Our results also indicated that EDP caused a lowering of inhibin concentrations during pseudopregnancy and small numbers of follicles from 20 to 28 days after EDP. In contrast, EDP-induced pseudopregnancy appears to have no adverse effect on follicular development and subsequent ovulation following PGF2alpha administration.