Microsatellites for the gynogenetic Amazon molly, Poecilia formosa:: useful tools for detection of mutation rate, ploidy determination and overall genetic diversity
Microsatellites for the gynogenetic Amazon molly, Poecilia formosa:: useful tools for detection of mutation rate, ploidy determination and overall genetic diversity
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DOI：
10.1007/bf02728973
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发表时间：
2006-04-01
影响因子：
1.5
通讯作者：
Schartl, M
中科院分区：
文献类型：
--
作者：
Lampert, KP;Lamatsch, DK;Schartl, M
亚马逊莫莉出现在混合学校与P.墨西哥和P. latipinna。特别是福尔摩沙和墨西哥的幼鱼和雌鱼在形态上很难区分，因此我们有兴趣寻找工具来确定形态中间个体的种类。此外，倍性水平的快速测定对于许多关于父本渐渗的进化重要性的问题是重要的。因此，找到能够轻松区分二倍体和三体个体的遗传标记也很重要。微卫星标记已被证明在研究福尔摩沙三倍体克隆变异性和起源方面具有很高的价值（Lampert et al. 2005）。尽管克隆和分离微卫星标记需要相当大的努力（哈蒙德等，1998），但它们通常被认为是群体遗传学研究中最有力的工具（Jarne和Lagoda，1996）。对分离和克隆的一个合理的妥协是利用密切相关物种中相对高度保守的微卫星侧翼区（例如摩尔等人，1991）。这里我们
Amazon mollies occur in mixed schools with P. mexicana and with P. latipinna. As especially juveniles and females of P. formosa and P. mexicana are hard to distinguish morphologically we were interested in finding tools to determine the species of morphologically intermediate individuals easily. In addition, rapid determination of ploidy level is important for many questions concerning the evolutionary importance of paternal introgression. It is, therefore, also important to find genetic markers that will allow to distinguish between diploid and triploid individuals easily. Microsatellite markers have already been shown to be of high value for studies on the clonal variability and origin of triploid clones in P. formosa (Lampert et al. 2005). Despite the considerable effort necessary to clone and isolate microsatellite markers (Hammond et al. 1998) they are generally considered the most powerful tools for populationgenetic studies (Jarne and Lagoda 1996). A reasonable compromise to isolation and cloning is the exploitation of the relatively high conservation of microsatellite flanking regions in closely related species (eg Moore et al. 1991). Here we