Transgenic studies on the involvement of cytokinin and gibberellin in male development
Transgenic studies on the involvement of cytokinin and gibberellin in male development
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DOI：
10.1104/pp.102.018598
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发表时间：
2003-03-01
期刊：
影响因子：
7.4
通讯作者：
Ness, LA
中科院分区：
文献类型：
--
作者：
Huang, S;Cerny, RE;Ness, LA
许多植物激素在植物生长发育过程中相互作用。阐明这些不同激素在特定组织类型或发育阶段的作用一直是外源应用或组成表达研究的困难。因此，我们使用表达CKX1和gai的组织特异性启动子，分别参与氧化细胞分裂素降解和赤霉素（gibberellin， GA）信号转导的基因，研究细胞分裂素和GA在男性器官发育中的作用。CKX1基因在转基因玉米（Zea mays）生殖组织中的积累导致植株雄性不育。应用动蛋白和噻唑脲恢复了这些植物的雄性发育。同样，gai在烟草（Nicotiana tabacum）和拟南芥（Arabidopsis）的花药和花粉中的特异性表达导致了这些组织的败育。基因诱导的雄性不育表型通过外源应用动蛋白是可逆的。我们的研究结果提供了细胞分裂素和GA参与男性发育的分子证据，并支持了男性发育是由多种激素共同控制的假设。这些研究还提出了一种利用现有农用化学品在植物中产生可维持雄性不育的潜在方法，这将减少现有杂交作物制种的费用，并提供一种从传统非杂交作物中生产杂交品种的方法。
Numerous plant hormones interact during plant growth and development. Elucidating the role of these various hormones on particular tissue types or developmental stages has been difficult with exogenous applications or constitutive expression studies. Therefore, we used tissue-specific promoters expressing CKX1 and gai, genes involved in oxidative cytokinin degradation and gibberellin (GA) signal transduction, respectively, to study the roles of cytokinin and GA in male organ development. Accumulation of CKX1 in reproductive tissues of transgenic maize (Zea mays) resulted in male-sterile plants. The male development of these plants was restored by applications of kinetin and thidiazuron. Similarly, expression of gai specifically in anthers and pollen of tobacco (Nicotiana tabacum) and Arabidopsis resulted in the abortion of these respective tissues. The gai-induced male-sterile phenotype exhibited by the transgenic plants was reversible by exogenous applications of kinetin. Our results provide molecular evidence of the involvement of cytokinin and GA in male development and support the hypothesis that the male development is controlled in concert by multiple hormones. These studies also suggest a potential method for generating maintainable male sterility in plants by using existing agrochemicals that would reduce the expense of seed production for existing hybrid crops and provide a method to produce hybrid varieties of traditionally non-hybrid crops.