Construction of biodegradable and biocompatible AIE-active fluorescent polymeric nanoparticles by Ce(IV)/HNO3 redox polymerization in aqueous solution
Construction of biodegradable and biocompatible AIE-active fluorescent polymeric nanoparticles by Ce(IV)/HNO3 redox polymerization in aqueous solution
复制标题
通过水溶液中 Ce(IV)/HNO3 氧化还原聚合构建可生物降解和生物相容性 AIE 活性荧光聚合物纳米颗粒
DOI：
10.1016/j.msec.2017.04.064
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Wei Yen
中科院分区：
文献类型：
--
作者：
Yu Shengxian;Xu Dazhuang;Wan Qing;Liu Meiying;Tian Jianwen;Huang Qiang;Deng Fengjie;Wen Yuanqing;Zhang Xiaoyong;Wei Yen
聚合诱导发射（AIE）活性荧光聚合纳米粒子（FPNs）近年来在细胞成像、药物传递、疾病诊断和治疗等生物医学应用方面受到越来越多的关注。高效、简化、柔嫩的aie活性fpn的制备策略仍被热切追求，以促进治疗系统的发展。在本工作中，我们开发了一种在近室温下采用Ce(IV)/ hno3氧化还原聚合制备aie活性fpn的简便方法。由此制备的fpn（命名为PEG-PLC-1）由于超亮AIE染料（ph -alc）与可生物降解的PEG-PCL线状共聚物的偶联，具有独特的AIE特性、良好的水分散性、良好的生物相容性和可生物降解性。利用核磁共振（NMR）谱、傅里叶变换红外（FT-IR）谱、透射电子显微镜（TEM）、紫外可见光谱和荧光光谱仪证实了aie活性FPNs的成功制备。进一步研究了PEG-PLC-1 FPNs的细胞活力和细胞摄取行为，以确定其潜在的生物医学应用。结果表明，PEG-PLC-1 FPNs具有高水分散性、强发光性和低细胞毒性，在生物医学应用中具有很大的吸引力。更重要的是，制备aie活性生物可降解fpn的方法可以在相当简单的条件下进行（例如，低温，无金属催化剂，普通链转移剂和水溶液），并且专门用于制备有机溶解度较差的aie活性多糖。
Aggregation-induced emission (AIE) active fluorescence polymeric nanoparticles (FPNs) have recently received increasing interests for biomedical applications such as cell imaging, drug delivery, disease diagnosis and treatment. Fabricated strategies of AIE-active FPNs with high efficiency, simplification and tenderness are still passionately pursued to promote the development of theranostic systems. In this work, we develop a facile method for the preparation of AIE-active FPNs by adopting Ce(IV)/HNO3redox polymerization under near room temperature. Thus-prepared FPNs (named as PEG-PLC-1) possess unique AIE feature, great water dispersity, excellent biocompatibility and biodegradability because of the conjugation of ultra-bright AIE dye (PhE-alc) and biodegradable PEG-PCL linear copolymers. The1H nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), UV–Visible and fluorescence spectrometers were used to confirm the successful fabrication of AIE-active FPNs. Cell viability and cellular uptake behavior of PEG-PLC-1 FPNs were further investigated for their potential biomedical applications. Results demonstrated that PEG-PLC-1 FPNs are high water dispersity, intensive luminescence and low cytotoxicity, making them very attractive for biomedical applications. More importantly, the method for the fabrication of AIE-active biodegradable FPNs can be occurred under rather facile conditions (e.g., low temperature, free of metal catalysts, common chain transfer agent and aqueous solution) and are specially used for fabrication of AIE-active polysaccharides with poor organic solubility.