A novel N18TG2 x mesencephalon cell hybrid expresses properties that suggest a dopaminergic cell line of substantia nigra origin
A novel N18TG2 x mesencephalon cell hybrid expresses properties that suggest a dopaminergic cell line of substantia nigra origin
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DOI：
10.1523/jneurosci.12-09-03392.1992
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发表时间：
1992-09
期刊：
影响因子：
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通讯作者：
G. Crawford;WD Le;R.Glenn Smith;WJ Xie;E. Stefani;Sarit Appel
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文献类型：
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作者：
G. Crawford;WD Le;R.Glenn Smith;WJ Xie;E. Stefani;Sarit Appel
多巴胺能神经母细胞瘤是使用大鼠胚胎中脑细胞和小鼠神经母细胞瘤-神经胶质瘤细胞系N18 TG 2的体细胞融合衍生的。由此产生的种间杂种，命名为MES23.5，保持了稳定的表型和核型的连续培养期1年。混合表现出几个属性，表明父母的初级神经元起源于黑质。该细胞系含有酪氨酸羟化酶，其可通过生物化学和免疫学方法鉴定，并合成多巴胺，但不合成其他儿茶酚胺。另外，细胞系表达表观电压门控CA 2+通道，如通过高亲和力ω-芋螺毒素结合所测量。MES23.5 ω-芋螺毒素受体与成年大鼠中脑中发现的受体具有相似的亲和力类别。通过PN 200 -100配体结合测量，不存在二氢吡啶受体。在N18 TG 2父代中未发现这些属性。至少有三个神经元的功能，即酪氨酸羟化酶，多巴胺的合成，和ω-芋螺毒素受体的表达，定量升高后持续治疗与cAMP类似物。该细胞系表达了黑质多巴胺能神经元中发现的一系列复杂的神经特性，因此可能有助于阐明其细胞生物学的进一步细节。
A dopaminergic neuroblastoma was derived using somatic cell fusion of rat embryonic mesencephalon cells and the murine neuroblastoma-glioma cell line N18TG2. The resulting interspecies hybrid, named MES23.5, has retained a stable phenotype and karyotype for a continuous culture period of 1 year. The hybrid exhibits several properties that suggest that the parent primary neurons originated in the substantia nigra. The cell line contains tyrosine hydroxylase, which is identifiable both by biochemical and immunological methods and synthesizes dopamine, but no other catecholamine. Additionally, the cell line expresses apparent voltage-gated CA2+ channels as measured by high-affinity omega- conotoxin binding. The MES23.5 omega-conotoxin receptors are of similar affinity class to those found in adult rat mesencephalon. No dihydropyridine receptors, as measured by PN200–100 ligand binding, are present. None of these properties are found in the N18TG2 parent. At least three neuronal features, namely, tyrosine hydroxylase, dopamine synthesis, and omega-conotoxin receptor expression, are quantitatively elevated after sustained treatment with cAMP analogs. The cell line expresses a complex range of neural properties found in the dopaminergic neurons of the substantia nigra, and may therefore be useful elucidating further details of their cell biology.