PROPOSAL FOR USE OF STANDARDIZED METHODS FOR CHLOROPHYLL DETERMINATIONS IN ECOLOGICAL AND ECO-PHYSIOLOGICAL INVESTIGATIONS
PROPOSAL FOR USE OF STANDARDIZED METHODS FOR CHLOROPHYLL DETERMINATIONS IN ECOLOGICAL AND ECO-PHYSIOLOGICAL INVESTIGATIONS
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DOI：
10.1111/j.1399-3054.1974.tb03743.x
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发表时间：
1974-01-01
影响因子：
6.4
通讯作者：
LINDER, S
中科院分区：
文献类型：
--
作者：
LINDER, S
在文献中发现的叶绿素测定的各种方法，可能给出的叶绿素值相差多达20%。不仅溶剂和方程的选择，而且样品处理的程序和条件也可能是很大的误差源。本文给出了有关植物材料取样和储存的一些建议。叶绿素的提取和测定。描述了一种简单快速的叶绿素测定方法，并推荐作为大多数生态和生态生理学研究的合适方法，其中叶绿素决心已定该方法的原理是叶绿素的总量在色度计中测定，该色度计经校准用于叶绿素测量。从原型的“叶绿素计”的结果，并与分光光度测定进行比较。
The various methods for chlorophyll determination, found in the literature, may give chlorophyll values differing by as much as 20%. Not only the choice of solvent and equations but also the routines and conditions for processing of samples can be large sources of errors.In the present paper some recommendations are given concerning sampling and storage of plant material, extraction and determination of chlorophylls.A simple and fast method for chlorophyll determinations is described and recommended as a suitable method for most ecological and eco‐physiological investigations in which chlorophylls are determined. The principle of the method is that the total amount of chlorophylls is determined in a colorimeter calibrated for chlorophyll measurements. Results from a prototype of the “Chlorophyllometer” are presented and compared with spectrophotometric determinations.