Sequences regulating temporal poly(A) site switching in the adenovirus major late transcription unit.
Sequences regulating temporal poly(A) site switching in the adenovirus major late transcription unit.
复制标题
调节腺病毒主要晚期转录单位中时间性 Poly(A) 位点转换的序列。
DOI：
10.1128/mcb.11.12.5977-5984.1991
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发表时间：
1991
影响因子：
5.3
通讯作者：
Imperiale,MJ
中科院分区：
文献类型：
--
作者：
DeZazzo,JD;Falck-Pedersen,E;Imperiale,MJ
聚(A)位点选择的时间调控发生在腺病毒重组体中，腺病毒重组体编码具有两个聚(A)位点L1和L3的主要晚期转录单元的微型版本。利用缺失诱变技术，我们直接研究了在重组中调控poly(A)位点选择的强制作用元件。从这项工作中，我们得出了两个主要结论。首先，除了AAUAAA和L1 poly(A)位点的下游序列外，其他元件在感染期间对poly(A)位点的选择进行时间调控是必需的。其次，这些区域在感染期间以两种不同的模式起作用。在早期感染期间，这两个区域以一种附加的方式增强了L1聚(A)位点的选择，但在晚期感染期间，任何一个元素的删除都消除了聚(A)位点选择的开关。这项工作记录了核心聚(a)区域下游调控元件的第一个例子。
Temporal regulation of poly(A) site choice occurs in an adenovirus recombinant encoding a miniature version of the major late transcription unit with two poly(A) sites, L1 and L3. Using deletion mutagenesis, we have looked directly forcis-acting elements regulating poly(A) site choice in this recombinant. From this work, we draw two main conclusions. First, elements other than the AAUAAA and downstream sequences of the L1 poly(A) site are required for temporal regulation of poly(A) site choice during infection. Second, these regions function in two distinct modes during infection. The two regions enhance selection of the L1 poly(A) site in an additive manner during an early infection, but deletion of either element abolishes the switch in poly(A) site choice during a late infection. This work documents the first example of a regulatory element downstream of a core poly(A) region.