The diversity of archaea and bacteria in association with the roots of Zea mays L.
The diversity of archaea and bacteria in association with the roots of Zea mays L.
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DOI：
10.1007/s002480000087
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发表时间：
2001-04-01
影响因子：
3.6
通讯作者：
Triplett, EW
中科院分区：
文献类型：
--
作者：
Chelius, MK;Triplett, EW
研究了玉米根表面和根内部细菌和古细菌的多样性。被查设计了一对细菌16 SrDNA引物，通过PCR直接从玉米根中扩增细菌序列，排除真核生物和叶绿体DNA。通过大小分级，玉米的线粒体序列很容易从PCR扩增的细菌序列中分离。还评估了细菌群落的可培养组分，反映了与克隆文库不同的群落组成。通过培养获得的生物体和通过16 S rDNA直接PCR扩增鉴定的生物体之间的系统发育重叠为48%。在培养物收集物中仅发现4个细菌分裂，其代表27种细菌类型，而在克隆分析中鉴定了6个分裂，包括74种细菌类型，包括最初描述为黄石温泉中的新分裂水平谱系的OP 10候选分裂的成员。在培养物收集中的主要组是放线菌，并且在克隆文库内，α-变形菌占主导地位。玉米相关的变形菌的人口类似于一个典型的土壤群落中居住的特定的植物相关的细菌，如根瘤菌和Herbaspirillum相关的细菌类型的子集的变形菌人口。其他部门（OP 10和Acidobacterium）内的细菌类型的代表性表明，玉米根支持一个独特的细菌群落。古菌域内的多样性较低。在筛选的50个克隆中，鉴定出6种独特的序列类型，其中5种彼此高度相关（共享98%，序列同一性）。古菌序列聚类良好的引导支持附近的海洋组I（crenarchea）和海洋组II（euryarchea）未培养的古菌。结果表明，玉米支持一个不同的根相关的微生物群落组成的物种，第一次被描述为居民的植物根环境。
The diversity of bacteria and archaea associating on the surface and interior of maize roots (Zea mays L.) was investigated. A bacterial 16S rDNA primer was designed to amplify bacterial sequences directly from maize roots by PCR to the exclusion of eukaryotic and chloroplast DNA. The mitochondrial sequence from maize was easily separated from the PCR-amplified bacterial sequences by size fractionation. The culturable component of the bacterial community was also assessed, reflecting a community composition different from that of the clone library. The phylogenetic overlap between organisms obtained by cultivation and those identified by direct PCR amplification of 16S rDNA was 48%. Only 4 bacterial divisions were found in the culture collection, which represented 27 phylotypes, whereas 6 divisions were identified in the clonal analysis, comprising 74 phylotypes, including a member of the OP10 candidate division originally described as a novel division level lineage in a Yellowstone hot spring. The predominant group in the culture collection was the actinobacteria and within the clone library, the oc-proteobacteria predominated. The population of maize-associated proteobacteria resembled the proteobacterial population of a typical soil community within which resided a subset of specific plant-associated bacteria, such as Rhizobium- and Herbaspirillum-related phylotypes. The representation of phylotypes within other divisions (OP10 and Acidobacterium) suggests that maize roots support a distinct bacterial community. The diversity within the archaeal domain was low. Of the 50 clones screened, 6 unique sequence types were identified, and 5 of these were highly related to each other (sharing 98%, sequence identity). The archaeal sequences clustered with good bootstrap support near Marine group I (crenarchaea) and with Marine group II (euryarchaea) uncultured archaea. The results suggest that maize supports a diverse root-associated microbial community composed of species that for the first time have been described as inhabitants of a plant-root environment.