Juglanin inhibits IL-1β-induced inflammation in human chondrocytes
Juglanin inhibits IL-1β-induced inflammation in human chondrocytes
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DOI：
10.1080/21691401.2019.1657877
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发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Huo, Shousong
中科院分区：
文献类型：
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作者：
Chen, Xinxin;Zhang, Chengyong;Huo, Shousong
骨关节炎（OA）是与软骨细胞凋亡相关的最具特征的关节疾病之一。据报道，胡桃苷具有抗炎活性。本研究旨在评价胡桃苷对人OA软骨细胞的保护性抗炎作用。用胡桃苷（10、20和40 μ m）预处理人OA软骨细胞2小时，随后用IL-1 β刺激24小时。使用Griess方法测定一氧化氮（NO）的产生，并使用ELISA评估前列腺素E2（PGE 2）、基质金属蛋白酶-3、基质金属蛋白酶-9和基质金属蛋白酶-13（MMP-3、MMP-9和MMP-13）、TNE-α和IL-6。采用qRT-PCR和western blot方法检测诱导型一氧化氮合酶（iNOS）、环氧化酶2（考克斯-2）、具有血小板反应蛋白基序4和5的去整合素和金属蛋白酶（ADAMTS-4和ADAMTS-5）的表达。通过蛋白质印迹分析检测NF-κ B信号分子。结果表明，胡桃苷剂量依赖性地抑制IL-1 β诱导的PGE 2、NO、MMP 1、MMP 3、MMP 13、TNF-α和IL-6的产生。胡桃苷预处理可抑制IL-1 β诱导的考克斯-2、iNOS、ADAMTS-4和ADAMTS-5的表达。Western blot分析显示胡桃苷抑制IL-1 β诱导的NE-κ B活化。综上所述，我们发现胡桃苷通过调节NF-κ B信号传导抑制IL-1 β诱导的炎症。胡桃苷有可能作为治疗OA的药物。
Osteoarthritis (OA) is one of the most characterized joint diseases associated with chondrocyte apoptosis. Juglanin has been reported to have anti-inflammation activity. This study aimed to evaluate the protective anti-inflammatory effects of juglanin in human OA chondrocytes. Human OA chondrocytes were pretreated with juglanin (10, 20 and 40 mu m) for 2 h and subsequently stimulated with IL-1 beta for 24 h. Nitric oxide (NO) production was determined using the Griess method and prostaglandin E2 (PGE2), matrix metalloproteinase-3, -9 and -13 (MMP-3, MMP-9 and MMP-13), TNE-alpha, and IL-6 were assessed using ELISA. The expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), a disintegrin and metalloproteinase with thrombospondin motifs-4 and -5 (ADAMTS-4 and ADAMTS-5) were detected by qRT-PCR and western blot analysis. NF-kappa B signalling molecules were detected by western blot analysis. The results showed that juglanin dose-dependently suppressed PGE2, NO, MMP1, MMP3, MMP13, TNF-alpha and IL-6 production induced by IL-1 beta. The expression of COX-2, iNOS, ADAMTS-4 and ADAMTS-5 induced by IL-1 beta were also suppressed by juglanin pretreatment. Western blot analysis showed that juglanin suppressed IL-1 beta-induced NE-kappa B activation. Taken together, we found that juglanin inhibits IL-1 beta-induced inflammation through the regulation of NF-kappa B signalling. Juglanin might be used as a therapeutic agent for treating OA.