Identifying New Components Participating in the Secondary Cell Wall Formation of Vessel Elements in Zinnia and Arabidopsis
Identifying New Components Participating in the Secondary Cell Wall Formation of Vessel Elements in Zinnia and Arabidopsis
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DOI：
10.1105/tpc.108.059154
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发表时间：
2009-04-01
期刊：
影响因子：
11.6
通讯作者：
Demura, Taku
中科院分区：
文献类型：
--
作者：
Endo, Satoshi;Pesquet, Edouard;Demura, Taku
木质部导管分子是中空的细胞单元，其端对端组装以在整个植物体中形成连续的导管;木质部导管通过木质部分子的增强的次生细胞壁（SCW）来加强。本工作旨在利用百日菊分化细胞培养的体外细胞培养模型和拟南芥体内细胞培养的模型来阐明未知因素在木质部导管分化中的作用。基于百日菊系统中的RNA干扰（RNAi）筛选，选择了导管元件分化相关6（TED 6）和TED 7。RNAi减少TED 6和7延迟了气管元件（TE）的分化，而TED 6和7的共过表达增加了培养的百日菊细胞中的TE分化。拟南芥TED 6和7在幼苗导管分子分化中优先表达。根导管元件的异常SCW形成由单独的At TED 7的瞬时RNAi诱导，并通过抑制TED 6和7来增强。TED 6和SCW相关的纤维素合成酶复合物的亚基之间的蛋白质-蛋白质相互作用被证明。我们的策略已经成功地发现了两个新的组成部分，在SCW的形成，并打开了大门，深入分析其分子功能。
Xylem vessel elements are hollow cellular units that assemble end-to-end to form a continuous vessel throughout the plant body; the xylem vessel is strengthened by the xylem elements' reinforced secondary cell walls (SCWs). This work aims to unravel the contribution of unknown actors in xylem vessel differentiation using the model in vitro cell culture system of Zinnia elegans differentiating cell cultures and the model in vivo system of Arabidopsis thaliana plants. Tracheary Element Differentiation-Related6 (TED6) and TED7 were selected based on an RNA interference (RNAi) screen in the Zinnia system. RNAi reduction of TED6 and 7 delayed tracheary element (TE) differentiation and co-overexpression of TED6 and 7 increased TE differentiation in cultured Zinnia cells. Arabidopsis TED6 and 7 were expressed preferentially in differentiating vessel elements in seedlings. Aberrant SCW formation of root vessel elements was induced by transient RNAi of At TED7 alone and enhanced by inhibition of both TED6 and 7. Protein-protein interactions were demonstrated between TED6 and a subunit of the SCW-related cellulose synthase complex. Our strategy has succeeded in finding two novel components in SCW formation and has opened the door for in-depth analysis of their molecular functions.