Comparison of backscattered scanning electron microscopy and microradiography of secondary caries.
Comparison of backscattered scanning electron microscopy and microradiography of secondary caries.
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继发龋的背散射扫描电子显微镜和显微射线照相的比较。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
S. J. Marshall
中科院分区：
文献类型：
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作者：
G. Marshall;M. Staninec;Y. Torii;S. J. Marshall
龋损通常使用需要制备薄切片的光学显微镜和/或显微放射照相术来研究。背散射扫描电子显微镜（BSEM）已收到很少的关注，虽然它提供的信息与显微射线照相获得的类似，具有更高的分辨率的潜力。最近，显微镜已被引入，可用于研究潮湿或不导电的标本，提供研究标本的技术，而无需干燥或制备薄切片。本研究旨在确定显微放射照相术研究时继发性龋损是否具有与使用“湿”BSEM模式时相同的特征。显微放射照片是由人工龋系统中诱发继发龋的修复牙的薄片制成的。薄切片还通过BSEM研究，其中在样品室中具有分压以防止样品充电。比较两种方法的病变大小和形状。牙釉质中的损伤深度测量值相同;渗透到牙本质中的损伤似乎具有相似的大小和形状，但通过BSEM测量的损伤深度略大（配对t检验，p小于0.05）。这是由于龋釉质-牙本质界面处的裂纹可能在样本储存期间形成。表面釉质棒结构的变化和亚表面病变的发展是明显的。在龋坏牙本质中也有几个区域明显，表明在病变的深度中牙本质小管细节的丧失、小管的塌陷和病变的前进前部附近的高矿化区域。检查了几个额外的天然龋齿样本。它们显示了龋过程的典型结构特征。（250字处删节）
Carious lesions are usually studied using light microscopy and/or microradiography which require preparation of thin sections. Backscattered scanning electron microscopy (BSEM) has received little attention although it provides information similar to that obtained with microradiographs, with the potential for higher resolution. Recently, microscopes have been introduced that can be used to study wet or nonconducting specimens, offering techniques for studying specimens without desiccation or preparation of thin sections. This investigation sought to determine if secondary carious lesions have the same characteristics when studied by microradiography as when using the "wet" BSEM mode. Microradiographs were made of thin sections from restored teeth with secondary caries induced in an artificial caries system. The thin sections were also studied by BSEM with a partial pressure in the specimen chamber to prevent specimen charging. Comparisons of the lesion size and shape were made using the two methods. Lesion depth measurements in enamel were the same; lesions that penetrated into dentin appeared to be of similar size and shape, but lesion depths measured by BSEM were slightly greater (paired t-test, p less than .05). This was a result of cracks at the carious enamel-dentin interface that probably developed during storage of the samples. Variations in the surface enamel rod structure and the development of subsurface lesions were apparent. Several zones were also apparent in the carious dentin, demonstrating loss of dentinal tubule detail in the depth of the lesion, collapse of tubules, and hypermineralized regions near the advancing front of the lesion. Several additional samples of natural carious teeth were examined. They demonstrated the characteristic structural features of the carious process.(ABSTRACT TRUNCATED AT 250 WORDS)