The expression of heat shock protein 60 in myocardium of patients with chronic atrial fibrillation
The expression of heat shock protein 60 in myocardium of patients with chronic atrial fibrillation
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DOI：
10.1007/s003950200019
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发表时间：
2002-05-01
影响因子：
9.5
通讯作者：
Schumacher, B
中科院分区：
文献类型：
--
作者：
Schäfler, AE;Kirmanoglou, K;Schumacher, B
目的心肌细胞通过表达不同的热休克蛋白(HSP)来应对应激。热休克蛋白60是由各种应激因素诱导的。本研究旨在探讨热休克蛋白60(HSP60)在心房颤动(房颤)中的表达。方法取14例择期心脏手术患者的右房标本，立即冰冻于液氮中。慢性房颤8例，窦性心律6例。用SDS-PAGE、Western印迹法检测HSP60蛋白水平，并根据肌动蛋白的免疫反应条带进行光密度测定。结果在慢性房颤患者的心肌标本中，我们发现HSP60的表达比窦性心律患者的心房心肌高2.5倍以上。结论慢性心房颤动时HSP60表达上调。
Objective Cardiomyocytes respond to stress with the expression of different heat shock proteins (HSP). HSP60 is induced by various stress factors. The aim of this study was to investigate the expression of HSP60 in human atrial fibrillation (AF). Method Right atrial samples from 14 patients undergoing elective cardiac surgery were excised and immediately frozen in liquid nitrogen. Eight patients had chronic AF and six patients were in sinus rhythm. The HSP60 protein level was determined by SDS-PAGE, Western blot and quantified by optical densitometry according to the immunoreactive bands of actin. Results In myocardial samples from patients with chronic AF, we found a more than 2.5-fold increase in HSP60 expression compared to atrial myocardium of patients in sinus rhythm. Conclusion This result indicates an up regulation of HSP60 in response to chronic atrial fibrillation.