Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS
Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS
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DOI：
10.1186/s13059-020-01969-6
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发表时间：
2020-03-03
期刊：
影响因子：
12.3
通讯作者：
Song, Chun-Xiao
中科院分区：
文献类型：
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作者：
Liu, Yibin;Cheng, Jingfei;Song, Chun-Xiao
我们提出了长读Tet-assisted吡啶硼烷测序（lrTAPS）的DNA甲基化和羟甲基化的区域高达10 kb的从纳克级输入的靶向碱基分辨率测序。与Oxford Nanopore和PacBio单分子实时（SMRT）测序兼容，lrTAPS检测甲基化的准确性与短读Illumina测序相当，但具有长距离表观遗传定相。我们应用lrTAPS对小鼠胚胎干细胞中难以定位的区域进行测序，并鉴定整合的B型肝炎病毒基因组中不同的甲基化事件。
We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome.