A DISTINCT PICORNAVIRUS GROUP IDENTIFIED BY SEQUENCE-ANALYSIS
A DISTINCT PICORNAVIRUS GROUP IDENTIFIED BY SEQUENCE-ANALYSIS
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DOI：
10.1073/pnas.89.18.8847
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发表时间：
1992-09-15
影响因子：
11.1
通讯作者：
STANWAY, G
中科院分区：
文献类型：
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作者：
HYYPIA, T;HORSNELL, C;STANWAY, G
虽然埃可病毒22目前被归类为小核糖核酸病毒家族肠病毒组的成员，但据报道，与该组的其他代表相比，它具有特殊的生物学特性。我们已经确定了埃可病毒22（哈里斯株）基因组的完整核苷酸序列，它似乎与所有其他研究的小核糖核酸病毒有显著不同。然而，基因组的组织[7339个核苷酸，不包括聚(A)链]与先前测序的小核糖核酸病毒相似。该基因组包括一个5'非翻译区，与阿弗索病毒和心脏病毒相比，该区域相对保守，随后是一个编码2180个氨基酸长的多蛋白的开放阅读框。通过直接测序确定了衣壳多肽VP1和VP3的氨基末端，并通过与其他小核糖核酸蛋白的比较预测了该多蛋白的其他蛋白水解裂解位点。埃可病毒22多肽与其他小核糖核酸病毒相应蛋白的氨基酸特性在14-35%范围内，与5种小核糖核酸病毒组（肠病毒、犀牛病毒、心脏病毒、阿弗索病毒和肝病毒）的代表所见百分比相似。我们的结果表明，埃可病毒22属于一个独立的小核糖核酸病毒群。
Although echovirus 22 is presently classified as a member of the enterovirus group in the family of picornaviruses, it has been reported to have exceptional biological properties when compared with other representatives of the group. We have determined the complete nucleotide sequence of the echovirus 22 (Harris strain) genome, which appears to be significantly different from all the other studied picornaviruses. However, the organization of the genome [7339 nucleotides, excluding the poly(A) tract] is similar to that of previously sequenced picornaviruses. This genome includes a 5' untranslated region, relatively well-conserved when compared with aphtho- and cardioviruses, followed by an open reading frame coding for a 2180-amino acid-long polyprotein. The amino termini of capsid polypeptides VP1 and VP3 were determined by direct sequencing, and the other proteolytic cleavage sites in the polyprotein were predicted by comparison with other picornavirus proteins. The amino acid identities of echovirus 22 polypeptides with the corresponding proteins of other picornaviruses are in the 14-35% range, similar to those percentages seen when representatives of the five picornavirus groups (entero-, rhino-, cardio-, aphtho-, and hepatoviruses) are compared. Our results suggest that echovirus 22 belongs to an independent group of picornaviruses.