STUDY OF FINE-STRUCTURAL CHANGES IN CARTILAGE-TO-BONE TRANSITION WITHIN DEVELOPING CHICK VERTEBRA
STUDY OF FINE-STRUCTURAL CHANGES IN CARTILAGE-TO-BONE TRANSITION WITHIN DEVELOPING CHICK VERTEBRA
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DOI：
10.1002/aja.1001400402
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发表时间：
1974-01-01
影响因子：
--
通讯作者：
LOW, FN
中科院分区：
文献类型：
--
作者：
CRISSMAN, RS;LOW, FN
在孵育9-16天时处死48只鸡胚。从第四或第五颈椎获取组织，浸入Karnovsky液中，在四氧化锇中后固定，在乙醇中脱水，用乙酸双氧铀乙醇溶液“整块”染色，并包埋在Epon 812中。按照头侧至尾侧顺序对椎骨进行定位，以进行横截面切片。薄切片用铀酰和铅盐溶液染色，用三磷酸钙饱和，以防止脱钙。软骨椎体内的软骨细胞发生在不同的退变阶段，没有有序的排列。可逆和不可逆的阶段被发现在软骨吸收前沿。约12.5天，细胞间基质中出现电子透明类骨质和矿化。快速侵入的血管形成高度可变的吸收前沿和不规则的骨髓腔。与伴随细胞的captured边界在前面，但其他地方开放的毛细血管允许血液元素与软骨直接接触。破软骨细胞与小面积的钙化软骨有关。在约14天时，成骨细胞在吸收前沿形成小梁，所述成骨细胞将骨类骨质存款在未钙化的软骨基质上。基质被侵蚀了。游离的骨小梁没有钙化的软骨基质的核心仍然存在。在骺板和椎体的发育生长过程之间的基本差异可能源于后者的吸收前端的大量的未钙化的软骨基质。
Forty‐eight chick embryos were killed at 9–16 days of incubation age. Tissue was obtained from the fourth or fifth cervical vertebra, immersed in Karnovsky's fluid, post‐fixed in osmium tetroxide, dehydrated in ethanol, stained “en bloc” with uranyl acetate in ethanol, and embedded in Epon 812. Vertebrae were oriented for cross‐sectional microtomy in cephalic to caudal sequence. Thin sections were stained with uranyl and lead salt solutions saturated with tribasic calcium triphosphate to prevent decalcification.Chondrocytes within the cartilaginous vertebral body occur in various stages of degeneration without orderly arrangement. Both reversible and irreversible stages are found at the cartilaginous resorption front. Electron‐lucent osteoid and mineralization appear in the intercellular matrix at about 12.5 days. Rapidly invading blood vessels form a highly variable resorption front and irregular marrow cavity. Capillaries with accompanying cells border on the front, but else‐where open capillaries allow blood elements to be in direct contact with cartilage. Chondroclasts are associated with small areas of calcified cartilage. At about 14 days trabeculae are formed at the resorption front by osteoblasts which deposit bone osteoid on uncalcified cartilaginous matrix. The matrix is eroded away. A free trabecula of bone without a core of calcified cartilaginous matrix remains.Basic differences between developmental growth processes in the epiphyseal plate and vertebral body may stem from the large amount of uncalcified cartilaginous matrix at the latter's resorption front.