Molecular assays reveal the presence of Theileria spp. and Babesia spp. in Asian water buffaloes (Bubalus bubalis, Linnaeus, 1758) in the Amazon region of Brazil
Molecular assays reveal the presence of Theileria spp. and Babesia spp. in Asian water buffaloes (Bubalus bubalis, Linnaeus, 1758) in the Amazon region of Brazil
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DOI：
10.1016/j.ttbdis.2016.05.009
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发表时间：
2016-01-01
影响因子：
3.2
通讯作者：
Ribeiro, Mucio F. B.
中科院分区：
文献类型：
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作者：
Silveira, Julia A. G.;de Oliveira, Cairo H. S.;Ribeiro, Mucio F. B.
巴西大约50%的水牛群位于巴西北部的帕拉州。在一些地方，牛和水牛一起生活和放牧，因此，水牛的病原体可能会威胁到牛的健康，反之亦然。因此，了解水牛的病原体对于保持健康的牲畜是必不可少的。泰勒氏原虫和巴贝斯虫寄生虫在亚洲水牛中引起的临床疾病并不常见，尽管这些动物可能充当水库宿主，在水牛身上检测这些血寄生虫与在牛身上一样重要。在巴西，有关血寄生虫感染水牛的研究很少。本研究的目的是通过套式聚合酶链式反应和系统发育分析，研究巴西亚马孙地区PATH州亚洲水牛体内是否存在锥形虫寄生虫。从308头明显健康的水牛的血液样本中提取DNA，扩增出18S rRNA基因及其完整区域，这些水牛是在巴西帕斯州的六种特性上饲养的。水牛标本中泰氏杆菌阳性率为4.2%(13/308)，牛巴贝斯虫阳性率为3.6%(11/308)，二重巴贝斯虫阳性率为1%(3/308)。在评估的房产中，有50%(3/6)的动物被检测到泰勒氏杆菌。系统发育分析表明，本研究检测到的泰勒虫与布氏泰勒虫、东方泰勒虫和中华泰勒虫亲缘关系较近。据我们所知，这是美洲亚洲水牛中泰勒氏菌的首次报告。大多数泰勒氏阳性水牛(11/13)属于这样一种属性，即有动物表现不明病因的淋巴增生性疾病的历史。因此，目前的研究表明，这种疾病可能与泰勒氏菌在该属性的感染有关。我们的结果为从水牛传播到牛的血寄生虫的分布和生物学方面提供了新的见解。(C)2016年爱思唯尔股份有限公司。版权所有。
Approximately 50% of buffalo herds in Brazil are located in Para state in northern Brazil. There are several properties where cattle and buffalo live and graze together, and thus, buffalo pathogens may threaten the health of cattle and vice versa. Therefore, knowledge of infectious agents of buffalo is essential for maintaining healthy livestock. Clinical disease caused by Theileria and Babesia parasites in the Asian water buffalo is not common, although these animals may act as reservoir hosts, and the detection of these hemoparasites in buffaloes is as important as it is in cattle. Studies of the infection of buffaloes by hemoparasites in Brazil are scarce. The objective of the present study was to investigate the occurrence of Piroplasmida parasites in Asian water buffaloes in the state of Path in the Amazon region of Brazil using nested PCR assays and phylogenetic analysis. The 18S rRNA gene and ITS complete region were amplified from DNA extracted from blood samples collected from 308 apparently healthy buffaloes bred on six properties in the state of Path, Brazil. The prevalence of positive buffalo samples was 4.2% (13/308) for Theileria spp., 3.6% (11/308) for Babesia bovis and 1% (3/308) for Babesia bigemina. Animals infected with Theileria were detected in 50% (3/6) of the assessed properties. Phylogenetic analyses indicated that the Theileria species detected in this study were closely related to Theileria buffeli, Theileria orientalis and Theileria sinensis. To our knowledge, this is the first report of Theileria in Asian water buffaloes in the Americas. The majority of Theileria-positive buffaloes (11/13) belong to a property that has a history of animals presenting lymphoproliferative disease of unknown etiology. Therefore, the present research suggests that this disorder can be associated with Theileria infection in this property. Our results provide new insights on the distribution and biological aspects of hemoparasites transmissible from buffaloes to cattle. (C) 2016 Elsevier GmbH. All rights reserved.