Recruitment of clathrin onto endosomes by the Tom1-Tollip complex
Recruitment of clathrin onto endosomes by the Tom1-Tollip complex
复制标题
DOI：
10.1016/j.bbrc.2005.12.156
复制
发表时间：
2006-03-03
影响因子：
3.1
通讯作者：
Nakayama, K
中科院分区：
文献类型：
--
作者：
Katoh, Y;Imakagura, H;Nakayama, K
Toni I（Myb 1的靶标）及其相关蛋白（Tom IL 1/Srcasm和Toni IL 2）构成蛋白质家族，并且共享N-末端VHS（Vps 27 p/Hrs/Stam）结构域和随后的GAT（GGA和Tom 1）结构域，这两个结构域在GGA家族蛋白中也是保守的。然而，C-末端一半在Tom I和GGA家族之间或甚至在Toni I和Toni I L1之间并不显著保守。我们以前已经表明，Toni I的GAT结构域与Tollip（Toll相互作用蛋白）相互作用，Tollip与内体相关，它招募Tom 1。我们在这里扩展了以前的数据，并表明，GAT结构域的汤姆IL I和汤姆IL 2也与Tollip相互作用，和所有的汤姆I家族蛋白质的C-末端区域与网格蛋白相互作用。此外，当与Tollip共表达时，所有的Toni I家族蛋白将网格蛋白募集到内体上。这些结果表明，与Tollip，Tom 1家族蛋白在招募网格蛋白到内体上发挥重要作用，并表明它们调节内体功能。(c)2006年爱思唯尔公司All rights reserved.
Toni I (target of Myb 1) and its related proteins (Tom I L1/Srcasm and Toni I L2) constitute a protein family and share an N-terminal VHS (Vps27p/Hrs/Stam) domain and a following GAT (GGA and Tom1) domain, both of which are also conserved in the GGA family proteins. However, the C-terminal half is not significantly conserved between the Tom I and GGA families or even between Toni I and Toni I L1. We have previously shown that the GAT domain of Toni I interacts with Tollip (Toll-interacting protein), which is associated with endosomes, to which it recruits Tom1. We here extend the previous data and show that the GAT domains of Tom I L I and Tom I L2 also interact with Tollip, and the C-terminal regions of all the Tom I family proteins interact with clathrin. Furthermore, when coexpressed with Tollip, all the Toni I family proteins recruite clathrin onto endosomes. These results indicate that, in conjunction with Tollip, Tom1 family proteins play an important role in recruiting clathrin onto endosomes and suggest that they modulate endosomal functions. (c) 2006 Elsevier Inc. All rights reserved.