CHOLESTERYL GLUCOSIDE IN CANDIDA-BOGORIENSIS
CHOLESTERYL GLUCOSIDE IN CANDIDA-BOGORIENSIS
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DOI：
10.1016/0005-2760(80)90213-1
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
KASTELICSUHADOLC, T
中科院分区：
文献类型：
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作者：
KASTELICSUHADOLC, T
用CHCl_3/CH_3OH/1 M HCl从新鲜和冷冻的C. bogoriensis显示存在甾醇葡糖苷。纯化产物并鉴定为β-胆甾醇葡糖苷它在甲醇HCl中水解，因此它是一种甾醇葡糖苷。采用高压液相色谱法对提取的胆甾醇葡萄糖苷进行定量。分离的和合成的β-的化学电离质谱比较胆固醇葡糖苷，发现相同。C.用酶（glusulase）弱化Bogoriensis，经匀浆和蔗糖密度梯度离心后，分离5层。只有1层的颗粒在用溶剂萃取后的TLC中显示存在胆固醇基葡糖苷。在同一层中，发现甾醇葡萄糖基转移酶的活性。
Extraction of lipids with CHCl3/CH3OH/1 M HCl from fresh and frozen cells of C. bogoriensis showed the presence of a steryl glucoside. The product was purified and identified as a .beta.-cholesteryl glucoside. It hydrolyzes in methanolic HCl and therefore it is a steryl glucoside. The quantitation of the extracted cholesteryl glucoside was pursued with high-pressure liquid chromatography. The chemical ionization mass spectra of the isolated and the synthesized .beta.-cholesteryl glucosides were compared and found identical. The cell wall of C. bogoriensis was weakened with enzyme (glusulase) and after homogenization and sucrose density gradient centrifugation, 5 layers separated. Only the pellets of 1 layer showed the presence of cholesteryl glucoside in TLC after extraction with solvents. In the same layer, the activity of sterol glucosyl transferase was found.