ALTERED IMMUNOEXPRESSION OF MICROGLIA AND MACROPHAGES AFTER MILD HEAD-INJURY
ALTERED IMMUNOEXPRESSION OF MICROGLIA AND MACROPHAGES AFTER MILD HEAD-INJURY
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DOI：
10.1089/neu.1995.12.53
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发表时间：
1995-02-01
影响因子：
4.2
通讯作者：
NOBLE, LJ
中科院分区：
文献类型：
--
作者：
AIHARA, N;HALL, JJ;NOBLE, LJ
在这项研究中，我们研究了小胶质细胞和巨噬细胞的时间反应，以轻度脑损伤的大鼠。小胶质细胞和巨噬细胞通过其独特的形态学和免疫表型进行鉴定。对于后者，分别使用针对0X42和ED 1的抗体来定义小胶质细胞和巨噬细胞。虽然轻度脑损伤后脑巨噬细胞的形态没有变化，但小胶质细胞的形态发生了显着变化。小胶质细胞体较大，细胞突起呈树枝状。头部损伤后，某些群体的巨噬细胞和小胶质细胞的免疫染色更强烈。到伤后3天，这些强染色的细胞在脑中表现出特征性的分布。在丘脑、海马、外侧和内侧膝状体以及黑质中检测到显著染色的小胶质细胞。强染色的巨噬细胞主要位于撞击部位附近的皮质和蛛网膜下腔。伤后7天，强烈免疫染色的巨噬细胞和小胶质细胞广泛分布在整个受伤的皮质。这些结果表明，小胶质细胞和巨噬细胞对轻度头部损伤敏感。巨噬细胞群体的早期变化与受损最严重的组织更直接相关，可能反映了这些细胞从蛛网膜下腔或穿过受损的血脑屏障的迁移。在没有明显神经元细胞损伤的区域中早期广泛的小胶质细胞反应表明这些细胞对在轻度创伤的大脑中表达的更微妙的因子有反应。
In this study we examined the temporal response of microglia and macrophages to mild head injury in the rat. Microglia and macrophages were identified by their distinct morphology and by immunophenotype. With regard to the latter, antibodies to OX42 and ED1 were used to define microglia and macrophages, respectively. Although there was no change in the morphology of brain macrophages after mild head injury, the morphology of microglia was dramatically altered. Microglial cell bodies appeared larger with a more elaborate arborization of cellular processes. After head injury certain populations of macrophages and microglia were more intensely immunostained. By 3 days postinjury these intensely stained cells exhibited a characteristic distribution in the brain. Prominently stained microglia were detected in the thalamus, hippocampus, lateral and medial geniculate body, and the substantia nigra. Intensely stained macrophages were located primarily in tbe cortex and subarachnoid space adjacent to the site of impact. By 7 days postinjury intensely immunostained macrophages and microglia were widespread throughout the injured cortex. These results demonstrate that microglia and macrophages are sensitive to mild head injury. Early changes in the macrophage population are more directly correlated with the most damaged tissue and may reflect migration of these cells from either the subarachnoid space or across the damaged blood-brain barrier. The early widespread microglial response in regions exhibiting no overt neuronal cell damage suggests that these cells are responding to more subtle factor(s) that are expressed in the mildly traumatized brain.