Dense fimbrial meshwork enhances Porphyromonas gingivalis adhesiveness:: a scanning electron microscopic study
Dense fimbrial meshwork enhances Porphyromonas gingivalis adhesiveness:: a scanning electron microscopic study
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DOI：
10.1111/j.1600-0765.2006.00922.x
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发表时间：
2007-04-01
影响因子：
3.5
通讯作者：
Morita, M.
中科院分区：
文献类型：
--
作者：
Hongo, H.;Takano, H.;Morita, M.
背景和目的：本研究的目的是确定牙龈卟啉单胞菌的菌毛功能在plaque formation.Material和Methods：我们用扫描电子显微镜检查聚集体和hemaggregates的菌毛丰富的ATCC 33277（父母）和菌毛穷人OZ6301 C（PGMA基因敲除，突变体）菌株牙龈卟啉单胞菌。结果：牙龈卟啉单胞菌聚集体由菌链和菌簇组成。从亲本菌株细胞伸出的丰富的菌毛倾向于聚集并在细菌细胞之间形成密集的网络。相比之下，突变株的细胞投射更少的菌毛和网络是松散的。包括亲本菌株细胞的血聚集体含有粘附于红细胞的分离的致密菌毛网。包含突变株细胞的血细胞聚集体包括细菌链和簇，其通过比亲本菌株更短的菌毛粘附于红细胞。亲株的血凝效价是突变株的10倍，而每细胞的菌毛数仅为突变株的2倍。结论：密集的菌毛网对牙龈卟啉单胞菌的血凝有显著的增强作用。因此，牙龈卟啉单胞菌的毒力因存在丰富的菌毛而增加。
Background and Objective: The aim of this study was to determine how the fimbriae of Porphyromonas gingivalis function in plaque formation.Material and Methods: We used scanning electron microscopy to examine aggregates and hemaggregates of fimbria-rich ATCC33277 (parent) and fimbra-poor OZ6301C (pgmA-knockout, mutant) strains of P. gingivalis. We also assessed the hemagglutination activity of the two strains as an indicator of P. gingivalis adhesiveness.Results: Aggregates of P. gingivalis were composed of bacterial chains and clusters. Rich fimbriae projecting from cells of the parent strain tended to bunch and form a dense meshwork among bacterial cells. In contrast, cells of the mutant strain projected fewer fimbriae and the meshwork was looser. Hemaggregates including cells of the parent strain contained a detached, dense fimbrial meshwork that adhered to erythrocytes. Hemaggregates comprising cells of the mutant strain included bacterial chains and clusters that adhered to erythrocytes by shorter fimbriae than those of the parent strain. The hemagglutination titer of the parent strain was 10-fold higher than that of the mutant strain, although the number of fimbriae per cell of the parent strain was only double that of the mutant strain.Conclusion: The results indicate that P. gingivalis adhesiveness is prominently enhanced by the dense fimbrial meshwork. Thus, the virulence of P. gingivalis is increased by the presence of rich fimbriae.